组织中模式识别受体的功能表达与女性生殖管道。
Functional expression of pattern recognition receptors in tissues of the human female reproductive tract.
机构信息
Department of Immunology and Microbiology, Dartmouth Medical School, Lebanon, NH 03756, USA.
出版信息
J Reprod Immunol. 2009 Jun;80(1-2):33-40. doi: 10.1016/j.jri.2008.12.004. Epub 2009 Apr 29.
Abstract
The human female reproductive tract (FRT) must balance the requirements of procreation with the demands of protection from pathogen invasion. We hypothesize that the FRT expresses functional pattern recognition receptors (PRRs), including Toll-like receptors (TLRs) and nucleotide-binding oligomerization domain (NOD) proteins that may mediate these tasks. Expression of PRRs was evaluated in FRT tissues by RT-PCR. PRR function within FRT tissue cells was determined by CXCL8 (IL-8) production in response to treatment with PRR agonists. We now report that TLRs7-9 are expressed in Fallopian tube, uterine endometrium, cervix and ectocervix, while TLR10 expression is restricted to Fallopian tube. NOD1 and NOD2 and the signal transducer RICK were detected in all FRT tissues. Stimulation of FRT tissue cells with PRR ligands resulted in secretion of CXCL8. Results of these studies indicate that PRRs are functionally expressed in FRT tissues, and suggest that these receptors mediate microbial recognition and immune defense in the reproductive tract.
摘要
人类女性生殖道（FRT）必须平衡生育需求和防止病原体入侵的需求。我们假设 FRT 表达功能性模式识别受体（PRR），包括 Toll 样受体（TLR）和核苷酸结合寡聚化结构域（NOD）蛋白，这些受体可能介导这些任务。通过 RT-PCR 评估 FRT 组织中 PRR 的表达。通过检测 PRR 激动剂处理后 CXCL8（IL-8）的产生来确定 FRT 组织细胞中 PRR 的功能。我们现在报告 TLRs7-9 在输卵管、子宫内膜、子宫颈和阴道外口表达，而 TLR10 的表达仅限于输卵管。NOD1 和 NOD2 以及信号转导物 RICK 存在于所有 FRT 组织中。PRR 配体刺激 FRT 组织细胞导致 CXCL8 的分泌。这些研究结果表明 PRR 在 FRT 组织中具有功能性表达，并表明这些受体在生殖道中介导微生物识别和免疫防御。
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