Research and Development Division, H-Plus Eco Ltd, BVC no. 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea.
Department of Bio & Environmental Technology, Division of Environmental & Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Nov;62(Pt 11):2703-2709. doi: 10.1099/ijs.0.029736-0. Epub 2012 Jan 6.
A Gram-stain-positive, strictly aerobic, short-rod-shaped, non-motile strain (designated MJ32(T)) was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. A polyphasic approach was applied to study the taxonomic position of strain MJ32(T). Strain MJ32(T) showed highest 16S rRNA gene sequence similarity to Gordonia hirsuta DSM 44140(T) (98.1%) and Gordonia hydrophobica DSM 44015(T) (97.0%); levels of sequence similarity to the type strains of other recognized Gordonia species were less than 97.0%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ32(T) belonged to the clade formed by members of the genus Gordonia in the family Gordoniaceae. The G+C content of the genomic DNA of strain MJ32(T) was 69.2 mol%. Chemotaxonomically, strain MJ32(T) showed features typical of the genus Gordonia. The predominant respiratory quinone was MK-9(H(2)), the mycolic acids present had C(56)-C(60) carbon atoms, and the major fatty acids were C(16:0) (34.6%), tuberculostearic acid (21.8%), C(16:1)ω7c (19.5%) and C(18:1)ω9c (12.7%). The peptidoglycan type was based on meso-2,6-diaminopimelic acid as the diagnostic diamino acid with glycolated sugars. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ32(T) is considered to represent a novel species of the genus Gordonia, for which the name Gordonia caeni sp. nov. is proposed. The type strain is MJ32(T) (=KCTC 19771(T)=JCM 16923(T)).
一株革兰氏阳性、严格需氧、短杆状、非运动的菌株（命名为 MJ32(T)）从韩国大田污水处理厂的污泥样本中分离得到。采用多相分类法研究了菌株 MJ32(T)的分类地位。菌株 MJ32(T)的 16S rRNA 基因序列与 Gordonia hirsuta DSM 44140(T)（98.1%）和 Gordonia hydrophobica DSM 44015(T)（97.0%）最为相似；与其他公认的 Gordonia 种的模式菌株的序列相似性均低于 97.0%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MJ32(T)属于 Gordonia 属在 Gordoniaceae 科中形成的分支。菌株 MJ32(T)的基因组 DNA G+C 含量为 69.2mol%。化学分类学上，菌株 MJ32(T)表现出典型的 Gordonia 属特征。主要的呼吸醌为 MK-9(H(2))，存在的分枝菌酸具有 C(56)-C(60)碳原子，主要脂肪酸为 C(16:0)（34.6%）、结核硬脂酸（21.8%）、C(16:1)ω7c（19.5%）和 C(18:1)ω9c（12.7%）。肽聚糖类型基于 meso-2,6-二氨基庚二酸作为诊断二氨基酸，具有糖基化糖。基于系统发育推断、脂肪酸图谱和其他表型特性，菌株 MJ32(T)被认为代表 Gordonia 属的一个新种，建议命名为 Gordonia caeni sp. nov.。模式菌株为 MJ32(T)（=KCTC 19771(T)=JCM 16923(T)）。
