Lee Chang-Muk, Weon Hang-Yeon, Kim Yoo-Jeong, Son Jung-A, Yoon Sang-Hong, Koo Bon-Sung, Kwon Soon-Wo
Korean Agricultural Culture Collection (KACC), Microbial Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1045-8. doi: 10.1099/ijs.0.002840-0.
A novel bacterium, designated strain 5YN1-3(T), was isolated from wetland peat collected from Yongneup, Korea. The bacterium was facultatively anaerobic, Gram-negative, yellow-coloured, rod-shaped, mesophilic and motile with one polar flagellum. The strain grew optimally at 30 degrees C, at pH 6.0-9.0 and with 0-1 % NaCl (w/v). 16S rRNA gene sequence analysis showed the highest similarity to the sequence from Aquitalea magnusonii TRO-001DR8(T), with 98.7 % sequence similarity. However, strain 5YN1-3(T) showed DNA-DNA relatedness of 43 % (40 % in a reciprocal experiment) with A. magnusonii LMG 23054(T). The strain contained summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c) and C(16 : 0) as major cellular fatty acids. On the basis of DNA-DNA relatedness and physiological and biochemical characterization, strain 5YN1-3(T) should be assigned to a novel species of the genus Aquitalea, for which the name Aquitalea denitrificans sp. nov. is proposed. The type strain is 5YN1-3(T) (=KACC 12729(T) =DSM 21300(T)).
从韩国龙陵采集的湿地泥炭中分离出一种新型细菌，命名为菌株5YN1-3(T)。该细菌为兼性厌氧、革兰氏阴性、黄色、杆状、嗜温菌，具一根极生鞭毛，能运动。该菌株在30℃、pH 6.0 - 9.0和0 - 1% NaCl（w/v）条件下生长最佳。16S rRNA基因序列分析表明，其与大阿奎塔利亚菌TRO-001DR8(T)的序列相似度最高，为98.7%。然而，菌株5YN1-3(T)与大阿奎塔利亚菌LMG 23054(T)的DNA - DNA相关性为43%（反向实验中为40%）。该菌株以特征性总和3（异-C(15 : 0) 2-OH和/或C(16 : 1)ω7c）和C(16 : 0)作为主要细胞脂肪酸。基于DNA - DNA相关性以及生理生化特征，菌株5YN1-3(T)应归为阿奎塔利亚菌属的一个新物种，为此提出新名称反硝化阿奎塔利亚菌（Aquitalea denitrificans sp. nov.）。模式菌株为5YN1-3(T)（=KACC 12729(T) =DSM 21300(T)）。
