Bhalerao Kaustubh D
Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 West Pennsylvania Ave, Urbana, IL 61801, USA.
Trends Biotechnol. 2009 Jun;27(6):368-74. doi: 10.1016/j.tibtech.2009.03.003. Epub 2009 May 4.
Engineering novel, reusable gene networks to provide greater control over cellular processes is one of the goals of the emerging discipline of synthetic biology. This article reviews the landmark literature pertaining to the development of synthetic gene networks, the engineering framework used to design and characterize them and the technological developments on the horizon that could potentially advance the field in new directions. As gene network engineering enters its second decade, an attempt is also made to outline the challenges in advancing this nascent field, especially with regard to the practical limitations of component reusability and reliability and the opportunities that present themselves in the development of novel gene expression controllers and single-cell biosensors.
构建新型、可重复使用的基因网络以更好地控制细胞过程是新兴的合成生物学学科的目标之一。本文回顾了与合成基因网络发展相关的里程碑式文献、用于设计和表征这些网络的工程框架，以及可能推动该领域朝着新方向发展的即将出现的技术进展。随着基因网络工程进入第二个十年，我们还试图概述推进这个新兴领域所面临的挑战，特别是在组件可重复使用性和可靠性的实际限制方面，以及在新型基因表达控制器和单细胞生物传感器开发中出现的机遇。
