Wang Steven Sheng-Shih, Yang Szu-Ming, Hsin Ai, Chang Yu-Kaung
Department of Chemical Engineering, National Taiwan University, 10617 Taipei, Taiwan, ROC.
Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, Taishan Dist., 24301 New Taipei, Taiwan, ROC.
Food Technol Biotechnol. 2018 Mar;56(1):40-50. doi: 10.17113/ftb.56.01.18.5456.
Polyacrylonitrile (PAN) nanofibrous membrane was prepared by an electrospinning technique. After heat treatment and alkaline hydrolysis, the weak ion exchange membrane was grafted with chitosan molecule and then covalently immobilized with a Cibacron Blue F3GA (CB). Fibre diameter, porosity and pore size of the membrane and immobilized dye density were characterized. Furthermore, the membrane was applied to evaluate the binding performance of lysozyme under various operating parameters (pH, chitosan mass per volume ratio, dye concentration, ionic strength and temperature) in batch mode. The experimental results were directly applied to purify lysozyme from chicken egg white by membrane chromatography. The results showed that the capture efficiency, recovery yield and purification factor were 90 and 87%, and 47-fold, respectively, in a single step. The binding capacity remained consistent after five repeated cycles of adsorption-desorption operations. This work demonstrates that the dye-affinity nanofibrous membrane holds great potential for purification of lysozyme from real feedstock.
采用静电纺丝技术制备了聚丙烯腈（PAN）纳米纤维膜。经过热处理和碱水解后，将弱离子交换膜用壳聚糖分子接枝，然后与汽巴克隆蓝F3GA（CB）共价固定。对膜的纤维直径、孔隙率、孔径和固定化染料密度进行了表征。此外，该膜还用于评估在分批模式下各种操作参数（pH值、壳聚糖质量体积比、染料浓度、离子强度和温度）下溶菌酶的结合性能。实验结果直接应用于通过膜色谱法从鸡蛋白中纯化溶菌酶。结果表明，在单一步骤中，捕获效率、回收率和纯化因子分别为90%、87%和47倍。经过五个重复的吸附-解吸操作循环后，结合容量保持一致。这项工作表明，染料亲和纳米纤维膜在从实际原料中纯化溶菌酶方面具有巨大潜力。
