Dieler R, Shehata-Dieler W E, Brownell W E
Department of Otolaryngology, Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196.
J Neurocytol. 1991 Aug;20(8):637-53. doi: 10.1007/BF01187066.
Isolated cochlear outer hair cells undergo rapid, force-generating length changes in response to electrical stimulation. The cellular mechanism responsible for electromotility and its structural substrate is not yet known. Salicylates reduce and block electromotility in vitro. Therefore, we exposed isolated outer hair cells from the guinea pig cochlea to various doses of sodium salicylate and evaluated both ultrastructural changes and responses to electrical stimulation. Following salicylate superfusion, the subsurface cisternae showed dilatation, vesiculation and a deviation from their normal, unfenestrated, axial orientation below the plasma membrane. These changes were time and dose dependent and reversible over a time course of about 30 min. Electromotility was blocked and showed recovery following the same time course as the salicylate-induced reversible structural changes. These results indicate that intact, unfenestrated subsurface cisternae are required for the optimal generation of electrically-induced motility in mammalian outer hair cells.
孤立的耳蜗外毛细胞在受到电刺激时会经历快速的、产生力的长度变化。负责电运动性的细胞机制及其结构基础尚不清楚。水杨酸盐在体外会降低并阻断电运动性。因此，我们将豚鼠耳蜗的孤立外毛细胞暴露于不同剂量的水杨酸钠中，并评估超微结构变化和对电刺激的反应。水杨酸盐灌注后，细胞膜下池出现扩张、囊泡化，并且偏离了其在质膜下方正常的、无窗孔的轴向排列。这些变化具有时间和剂量依赖性，并且在约30分钟的时间过程中是可逆的。电运动性被阻断，并在与水杨酸盐诱导的可逆结构变化相同的时间过程后显示恢复。这些结果表明，完整的、无窗孔的细胞膜下池是哺乳动物外毛细胞中电诱导运动性最佳产生所必需的。
