Gorjup Erwin, Danner Sandra, Rotter Nicole, Habermann Jens, Brassat Ute, Brummendorf Tim H, Wien Sascha, Meyerhans Andreas, Wollenberg Barbara, Kruse Charli, von Briesen Hagen
Department of Cell Biology and Applied Virology, Fraunhofer Institute for Biomedical Engineering, Ensheimer Strasse 48, D-66386 St. Ingbert, Germany.
Eur J Cell Biol. 2009 Jul;88(7):409-21. doi: 10.1016/j.ejcb.2009.02.187. Epub 2009 May 2.
Stem cells derived from pancreatic tissue are well characterized and exhibit a broad plasticity as they can differentiate beyond lineage boundaries into many cell types. The aim of this study was the comparative characterization of pancreatic stem cells with one other derivate of the embryonic foregut, namely salivary glands, for the existence of similar stem cell populations. The expression of stem cell markers as well as lineage-specific markers was detected by reverse transcription polymerase chain reaction, flow cytometry and immuncytochemical staining. The isolated cells from salivary glands and pancreas grew adherently in vitro and could be maintained for up to 55 and 46 population doublings, respectively. Cells from both tissues showed a comparable phenotype. They expressed different embryonic and adult stem cell markers and had the ability to differentiate spontaneously into cells representing the three embryonic germ layers. Additionally, the directed differentiation of glandular stem cells into the mesodermal lineage was achieved, yielding adipogenic, osteogenic and chondrogenic cells from salivary gland stem cells as well as osteogenic and chondrogenic cells from pancreatic stem cells. Here, we compared two stem cell populations from different glandular tissues which showed similar phenotypes and analogous properties. During embryonic development the two exocrine glands originate from the foregut, which might be the explanation for these intriguing resemblances.
源自胰腺组织的干细胞具有良好的特征，并且表现出广泛的可塑性，因为它们能够跨越谱系界限分化为多种细胞类型。本研究的目的是比较胰腺干细胞与胚胎前肠的另一种衍生物，即唾液腺，以确定是否存在相似的干细胞群体。通过逆转录聚合酶链反应、流式细胞术和免疫细胞化学染色检测干细胞标志物以及谱系特异性标志物的表达。从唾液腺和胰腺分离出的细胞在体外贴壁生长，分别可维持多达55和46次群体倍增。来自两种组织的细胞表现出可比的表型。它们表达不同的胚胎和成体干细胞标志物，并且有能力自发分化为代表三个胚胎胚层的细胞。此外，实现了腺干细胞向中胚层谱系的定向分化，从唾液腺干细胞产生脂肪生成、成骨和软骨生成细胞，以及从胰腺干细胞产生成骨和软骨生成细胞。在这里，我们比较了来自不同腺组织的两个干细胞群体，它们表现出相似的表型和类似的特性。在胚胎发育过程中，这两个外分泌腺起源于前肠，这可能是这些有趣相似性的解释。
