Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, LMU Munich, Veterinaerstrasse 13, 80539 Munich, Germany.
Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany.
Viruses. 2021 Oct 15;13(10):2080. doi: 10.3390/v13102080.
A real-time polymerase chain reaction (qPCR) is considered the gold standard for the laboratory diagnosis of canine parvovirus (CPV) infection but can only be performed in specialized laboratories. Several point-of-care tests (POCT), detecting CPV antigens in faeces within minutes, are commercially available. The aim of this study was to evaluate eight POCT in comparison with qPCR. Faecal samples of 150 dogs from three groups (H: 50 client-owned, healthy dogs, not vaccinated within the last four weeks; S: 50 shelter dogs, healthy, not vaccinated within the last four weeks; = 50 dogs with clinical signs of CPV infection) were tested with eight POCT and qPCR. Practicability, sensitivity, specificity, positive (PPV) and negative predictive values (NPV), as well as overall accuracy were determined. To assess the differences between and agreement among POCT, McNemar's test and Cohen's Kappa statistic were performed. Specificity and PPV were 100.0% in all POCT. Sensitivity varied from 22.9-34.3% overall and from 32.7-49.0% in group P. VetexpertRapidTestCPVAg had the highest sensitivity (34.3% overall, 49.0% group P) and differed significantly from the 3 POCT with the lowest sensitivities (FassisiParvo (27.7% overall, 36.7% group P), PrimagnostParvoH+K (24.3% overall, 34.7% group P), FASTestPARVOCard (22.9% overall, 32.7% group P)). The agreement among all POCT was at least substantial (kappa >0.80). A positive POCT result confirmed the infection with CPV in unvaccinated dogs, whereas a negative POCT result did not definitely exclude CPV infection due to the low sensitivity of all POCT.
实时聚合酶链反应（qPCR）被认为是犬细小病毒（CPV）感染的实验室诊断金标准，但只能在专门的实验室进行。有几种即时检测（POCT）可在数分钟内检测粪便中的 CPV 抗原，目前已商业化。本研究旨在比较 8 种 POCT 与 qPCR。从三个组（H：50 只来自客户的健康犬，未在过去四周内接种疫苗；S：50 只来自庇护所的健康犬，未在过去四周内接种疫苗； = 50 只患有 CPV 感染临床症状的犬）的 150 只狗的粪便样本中，用 8 种 POCT 和 qPCR 进行了检测。确定了实用性、敏感性、特异性、阳性预测值（PPV）和阴性预测值（NPV）以及总准确性。为了评估 POCT 之间的差异和一致性，进行了 McNemar 检验和 Cohen 的 Kappa 统计检验。在所有 POCT 中，特异性和 PPV 均为 100.0%。总体敏感性为 22.9-34.3%，组 P 为 32.7-49.0%。VetexpertRapidTestCPVAg 的敏感性最高（总体为 34.3%，组 P 为 49.0%），与敏感性最低的 3 种 POCT 有显著差异（FassisiParvo（总体为 27.7%，组 P 为 36.7%）、PrimagnostParvoH+K（总体为 24.3%，组 P 为 34.7%）、FASTestPARVOCard（总体为 22.9%，组 P 为 32.7%））。所有 POCT 之间的一致性至少为中等（kappa>0.80）。POCT 阳性结果证实了未接种疫苗的犬感染了 CPV，而由于所有 POCT 的敏感性都较低，POCT 阴性结果并不能完全排除 CPV 感染。
