Dragun Zrinka, Podrug Marijana, Raspor Biserka
Ruder Bosković Institute, Division for Marine and Environmental Research, PO Box 180, 10002 Zagreb, Croatia.
Comp Biochem Physiol C Toxicol Pharmacol. 2009 Aug;150(2):209-17. doi: 10.1016/j.cbpc.2009.04.011. Epub 2009 May 3.
The possible causes of the variability of gill metallothionein (MT) levels were studied on 182 specimens of 2- and 3-year old European chub (Squaliuscephalus L.) from the Sava River in Croatia. The most pronounced differences in MT levels were obtained between three sampling campaigns, and especially between periods with presumably different metabolic activities (April/May 2006 vs. September 2005 and 2006). Next to the probable influence of metabolic activity on MT level, the correlation analysis indicated a significant association between MTs and the fish size. Differences between males and females, as well as between mature and non-mature fish, were not observed in the young group of studied chub even in the spring reproductive season. Based on the analysis of the site-specific MT variability, it could be concluded that, under the conditions of low dissolved metal concentrations in the river water (as reported for the Sava River), MTs seem to be more affected by different biotic factors than by metal exposure. Therefore, MTs measured in this study were considered as the constitutive gill MT levels of young European chub. The constitutive MT ranges were defined separately for the season of lower metabolic rate (autumn: 1.31-2.00 mg g(-1)) and the season of higher metabolic activity (spring: 2.15-2.95 mg g(-1)).
对来自克罗地亚萨瓦河的182条2岁和3岁欧洲圆腹雅罗鱼（Squalius cephalus L.）样本，研究了鳃金属硫蛋白（MT）水平变化的可能原因。MT水平最显著的差异出现在三次采样活动之间，尤其是在代谢活动可能不同的时期（2006年4月/5月与2005年9月和2006年9月）。除了代谢活动对MT水平的可能影响外，相关性分析表明MT与鱼的大小之间存在显著关联。在所研究的幼龄雅罗鱼群体中，即使在春季繁殖季节，也未观察到雄性和雌性之间以及成熟和未成熟鱼之间的差异。基于对特定地点MT变异性的分析，可以得出结论，在河水溶解金属浓度较低的条件下（如萨瓦河所报道）MT似乎受不同生物因素的影响大于金属暴露。因此，本研究中测得的MT被视为幼龄欧洲圆腹雅罗鱼鳃的组成性MT水平。分别为代谢率较低的季节（秋季：1.31 - 2.00 mg g(-1)）和代谢活动较高的季节（春季：2.15 - 2.95 mg g(-1)）定义了组成性MT范围。
