Anandakumar P, Kamaraj S, Jagan S, Ramakrishnan G, Devaki T
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India.
Nat Prod Res. 2009;23(8):763-74. doi: 10.1080/14786410802604571.
In the present study, we have assessed the chemopreventive effect of capsaicin (CAP) on glucose metabolism with reference to blood glucose and liver glycogen levels, key glycolytic, and gluconeogenic enzymes along with electron transport chain (ETC) complexes during benzo(a)pyrene (B(a)P)-induced lung cancer in Swiss albino mice. B(a)P (50 mg kg(-1) body weight)-induced lung cancer animals showed marked decline in blood glucose levels, glycogen levels, elevations in the activities of key glycolytic enzymes (hexokinase, phosphoglucoisomerase and aldolase), and gluconeogenic enzymes (glucose-6-phosphatase and fructose-6-phosphatase) together with a decrease in the activities of ETC complexes. Supplementation of CAP (10 mg kg(-1) body weight) inhibited all the above alterations during lung cancer and restored near normalcy. Histochemical analysis by periodic acid Schiff's staining further confirmed the biochemical findings that highlighted the chemopreventive action of CAP during B(a)P-induced experimental lung tumourigenesis.
在本研究中，我们参照血糖和肝糖原水平、关键糖酵解酶和糖异生酶以及电子传递链（ETC）复合物，评估了辣椒素（CAP）对瑞士白化小鼠苯并(a)芘（B(a)P）诱导的肺癌过程中葡萄糖代谢的化学预防作用。B(a)P（50毫克/千克体重）诱导的肺癌动物血糖水平、糖原水平显著下降，关键糖酵解酶（己糖激酶、磷酸葡萄糖异构酶和醛缩酶）和糖异生酶（葡萄糖-6-磷酸酶和果糖-6-磷酸酶）活性升高，同时ETC复合物活性降低。补充CAP（10毫克/千克体重）可抑制肺癌期间的所有上述改变，并恢复至接近正常水平。过碘酸希夫染色的组织化学分析进一步证实了生化研究结果，突出了CAP在B(a)P诱导的实验性肺肿瘤发生过程中的化学预防作用。
