Harakeh Steve, Saleh Imane, Zouhairi Omar, Baydoun Elias, Barbour Elie, Alwan Nisreen
Biology Department, American University of Beirut, Beirut, Lebanon.
Sci Total Environ. 2009 Jun 15;407(13):4022-7. doi: 10.1016/j.scitotenv.2009.04.010. Epub 2009 May 9.
In this study Listeria monocytogenes (L. monocytogenes) was isolated from three traditionally consumed Lebanese dairy-based food products. One hundred and sixty four samples (45 samples of Baladi cheese, 36 samples of Shankleesh and 83 of Kishk) were collected from the Bekaa Valley in the Northeast region of Lebanon. Suspected Listeria colonies were selected and initially identified by using standard biochemical tests. Initial identification of the positive L. monocytogenes colonies was confirmed at the molecular level by Polymerase Chain Reaction (n=30) and the confirmed isolates were evaluated for their susceptibility to 10 commonly used antimicrobials. All of the 30 isolates were confirmed to be L. monocytogenes yielding a PCR product of approximately 660 base pairs (bp). L. monocytogenes was detected in 26.67%, 13.89% and 7.23% of the Baladi cheese, Shankleesh and Kishk samples, respectively. The highest resistance in L. monocytogenes isolates was noted against oxacillin (93.33%) followed by penicillin (90%). The results provide an indication of the contamination levels of dairy-based foods in Lebanon and highlight the emergence of multi-drug resistant Listeria in the environment.
在本研究中，从三种传统食用的黎巴嫩乳制品中分离出了单核细胞增生李斯特菌。从黎巴嫩东北部的贝卡谷地采集了164份样品（45份巴拉迪奶酪样品、36份香克利什样品和83份基什克样品）。挑选出疑似李斯特菌菌落，并通过标准生化试验进行初步鉴定。通过聚合酶链反应在分子水平上对阳性单核细胞增生李斯特菌菌落进行了初步鉴定（n = 30），并对确认的分离株进行了对10种常用抗菌药物的敏感性评估。所有30株分离株均被确认为单核细胞增生李斯特菌，产生了约660个碱基对（bp）的PCR产物。在巴拉迪奶酪、香克利什和基什克样品中，单核细胞增生李斯特菌的检出率分别为26.67%、13.89%和7.23%。单核细胞增生李斯特菌分离株对苯唑西林的耐药性最高（93.33%），其次是青霉素（90%）。这些结果表明了黎巴嫩乳制品的污染水平，并突出了环境中多重耐药李斯特菌的出现。
