Yeh Hsiao-Yun, Yates Marylynn V, Chen Wilfred, Mulchandani Ashok
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, United States.
Semin Cell Dev Biol. 2009 Feb;20(1):49-54. doi: 10.1016/j.semcdb.2009.01.012. Epub 2009 Feb 4.
Waterborne transmitted viruses pose a public health threat due to their stability in aquatic environment and the easy transmission with high morbidity rates at low infectious doses. Two major challenge of virus analysis include a lack of adequate information in infectivity and the inability to cultivate certain epidemiologically important viruses in vitro. The use of fluorescent probes in conjunction with fluorescence microscopy allows us to reveal dynamic interactions of the viruses with different cellular structures in living cells that are impossible to detect by immunological or PCR-based experiments. Real-time viral detection in vivo provides sufficient information regarding multiple steps in infection process at molecular level, which will be valuable for the prevention and control of viral infection.
水传播病毒因其在水生环境中的稳定性以及在低感染剂量下易于传播且发病率高，对公众健康构成威胁。病毒分析面临的两大挑战包括缺乏足够的感染性信息以及无法在体外培养某些具有重要流行病学意义的病毒。荧光探针与荧光显微镜结合使用，使我们能够揭示病毒与活细胞中不同细胞结构的动态相互作用，而这是免疫或基于PCR的实验无法检测到的。体内实时病毒检测在分子水平上提供了有关感染过程多个步骤的充分信息，这对于预防和控制病毒感染将是有价值的。
