Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, United States.
Environ Sci Technol. 2010 Dec 1;44(23):9030-5. doi: 10.1021/es102129d. Epub 2010 Nov 8.
We report development, characterization, and testing of chemiresistive immunosensors based on single polypyrrole (Ppy) nanowire for highly sensitive, specific, label free, and direct detection of viruses. Bacteriophages T7 and MS2 were used as safe models for viruses for demonstration. Ppy nanowires were electrochemically polymerized into alumina template, and single nanowire based devices were assembled on a pair of gold electrodes by ac dielectrophoretic alignment and anchored using maskless electrodeposition. Anti-T7 or anti-MS2 antibodies were immobilized on single Ppy nanowire using EDC-NHS chemistry to fabricate nanobiosensor for the detection of corresponding bacteriophage. The biosensors showed excellent sensitivity with a lower detection limit of 10(-3) plaque forming unit (PFU) in 10 mM phosphate buffer, wide dynamic range and excellent selectivity. The immunosensors were successfully applied for the detection of phages in spiked untreated urban runoff water samples. The results show the potential of these sensors in health care, environmental monitoring, food safety and homeland security for sensitive, specific, rapid, and affordable detection of bioagents/pathogens.
我们报告了基于单聚吡咯（Ppy）纳米线的化学电阻免疫传感器的开发、表征和测试，用于高度敏感、特异、无标记和直接检测病毒。噬菌体 T7 和 MS2 被用作病毒的安全模型进行演示。Ppy 纳米线通过电化学聚合到氧化铝模板中，并通过交流电介质电泳对准将单个纳米线基器件组装在一对金电极上，并使用无掩模电沉积固定。使用 EDC-NHS 化学将抗 T7 或抗 MS2 抗体固定在单个 Ppy 纳米线上，以制造用于检测相应噬菌体的纳米生物传感器。生物传感器表现出优异的灵敏度，在 10 mM 磷酸盐缓冲液中的检测下限为 10(-3) 噬菌斑形成单位 (PFU)，具有宽动态范围和出色的选择性。免疫传感器成功应用于检测未经处理的城市径流水样中的噬菌体。结果表明，这些传感器在医疗保健、环境监测、食品安全和国土安全方面具有潜在应用价值，可用于敏感、特异、快速和经济实惠地检测生物制剂/病原体。
