Register Karen B, Kunkle Robert A
Respiratory Diseases of Livestock Research Unit, USDA/Agricultural Research Service/National Animal Disease Center, P.O. Box 70, Ames, IA 50010, USA.
Avian Dis. 2009 Mar;53(1):50-4. doi: 10.1637/8388-070108-Reg.1.
Bordetella hinzii is commonly acquired from the respiratory tract of diseased poultry but is generally regarded as nonpathogenic in avian hosts because attempts to demonstrate disease following experimental infection of chickens and turkeys have failed. Recently, with the availability of highly specific DNA-based methods for identification of this agent, it was recognized that some isolates used in previous studies were misidentified at the time of their acquisition as Bordetella avium, B. avium-like, or Alcaligenes faecalis type II, including a subset reported to cause disease in turkey poults. In this study six strains of B. hinzii, genetically distinct and representing all known host species, were evaluated for their ability to colonize and cause disease in turkeys following intranasal administration. Although five strains were able to colonize the tracheas of turkey poults, only a subset induced clinical signs of disease, B. hinzii-specific antibodies, or tracheal lesions. The sixth isolate was undetectable in tracheal swabs obtained 1 or 2 weeks postinfection. Birds of this group displayed no clinical signs and minimal tracheal lesions. All remained B. hinzii seronegative. Three of the six strains, differing in their capacity to colonize and/or cause disease in turkeys, were used to infect chicks intranasally. Only one was able to colonize the trachea but did not induce tracheal lesions. No clinical signs of disease were observed in any chick. These results demonstrate that some strains of B. hinzii are virulent in turkey poults and may asymptomatically colonize chicks, and suggest this agent may be of concern to poultry producers.
欣氏博德特氏菌通常从患病家禽的呼吸道中获得，但一般被认为在禽类宿主中无致病性，因为对鸡和火鸡进行实验性感染后试图证明会引发疾病的尝试均告失败。最近，随着用于鉴定该病原体的高度特异性基于DNA方法的出现，人们认识到先前研究中使用的一些分离株在获取时被错误鉴定为鸟博德特氏菌、类鸟博德特氏菌或粪产碱菌II型，其中包括据报道会导致小火鸡发病的一个子集。在本研究中，对六株遗传上不同且代表所有已知宿主物种的欣氏博德特氏菌进行了评估，以确定它们经鼻内给药后在火鸡中定殖和引发疾病的能力。尽管五株菌能够在小火鸡的气管中定殖，但只有一个子集引发了疾病的临床症状、欣氏博德特氏菌特异性抗体或气管病变。第六个分离株在感染后1周或2周获得的气管拭子中无法检测到。该组的鸟类没有表现出临床症状，气管病变也很轻微。所有鸟类的欣氏博德特氏菌血清学检测均为阴性。六株菌中的三株在火鸡中定殖和/或引发疾病的能力不同，用于经鼻内感染雏鸡。只有一株能够在气管中定殖，但未引发气管病变。在任何雏鸡中均未观察到疾病的临床症状。这些结果表明，一些欣氏博德特氏菌菌株对小火鸡具有致病性，可能会无症状地定殖于雏鸡中，并表明该病原体可能是家禽生产者关注的问题。
