Moore Sara, Smyth W Franklin, Gault Victor A, O'Kane Edmund, McClean Stephen
The SAAD Centre for Pharmacy and Diabetes, Biomedical Sciences Research Institute, University of Ulster, Coleraine BT52 1SA, UK.
Rapid Commun Mass Spectrom. 2009 Jun;23(12):1747-55. doi: 10.1002/rcm.4063.
Arachnid venoms present a diverse and complex matrix for investigation, with their latent potential for innovative drug and pesticide design largely unrealised. The characterisation and quantification of selected low molecular mass compounds isolated from the crude venom of the Cobalt blue tarantula (Haplopelma lividum) were the objectives of this study. Fractionation of the crude venom was performed using reversed-phase high-performance liquid chromatography, with compound identification using both electrospray ionisation ion trap mass spectrometry and quadrupole time-of-flight mass spectrometry. Four compounds were identified, and quantification on a percentage dry weight basis was achieved by liquid chromatography/electrospray ionisation tandem mass spectrometry based on the formation of their corresponding product ions. Of these the most abundant component was glutamic acid, present at a level of 0.97%. Histamine and adenosine were detected at 0.14% and 0.10% dry weight, respectively, with the polyamine spermine noted in trace amounts at 0.002%. The limits of detection and quantification were established for each of the identified components. The fragmentation profile for histamine has also been proposed.
蜘蛛毒液是一个多样化且复杂的研究基质，其在创新药物和杀虫剂设计方面的潜在潜力很大程度上尚未实现。本研究的目的是对从钴蓝狼蛛（海南捕鸟蛛）粗毒液中分离出的选定低分子量化合物进行表征和定量。使用反相高效液相色谱法对粗毒液进行分离，并通过电喷雾电离离子阱质谱法和四极杆飞行时间质谱法对化合物进行鉴定。鉴定出了四种化合物，并基于其相应产物离子的形成，通过液相色谱/电喷雾电离串联质谱法实现了以干重百分比为基础的定量。其中含量最丰富的成分是谷氨酸，含量为0.97%。组胺和腺苷的干重含量分别为0.14%和0.10%，多胺精胺的含量为痕量，为0.002%。确定了每种已鉴定成分的检测限和定量限。还提出了组胺的碎裂图谱。
