Hamasaki N, Tomoda A, Harasaki H, Minakami S
J Biochem. 1977 May;81(5):1505-9.
Accumulation of exogenous phosphoenolpyruvate against the concentration gradient was observed when human red cells were incubated in an acidified isotonic sucrose medium. Fluoride increased the apparent accumulation by inhibition of the intracellular metabolic interconversion of the phosphate compound. The accumulation appeared to be specific for phosphoenolpyruvate and the accumulation rate for 3-phosphoglycerate, which has a molecular size and pKa similar to those of phosphoenolpyruvate, was less than one-tenth of the rate of phosphoenolpyruvate. Red cells incubated in the acidified sucrose medium tended to adhere to each other when examined with a scanning electron microscope.
当人红细胞在酸化的等渗蔗糖培养基中孵育时，观察到外源磷酸烯醇丙酮酸逆浓度梯度积累。氟化物通过抑制磷酸盐化合物的细胞内代谢互变增加了表观积累。这种积累似乎对磷酸烯醇丙酮酸具有特异性，而3-磷酸甘油酸（其分子大小和pKa与磷酸烯醇丙酮酸相似）的积累速率不到磷酸烯醇丙酮酸积累速率的十分之一。用扫描电子显微镜检查时，在酸化蔗糖培养基中孵育的红细胞倾向于相互粘附。
