Wang Yan, Cong Zhenna, Liu Qingfei, Ling Jiajun, Zhou Liling
Section of Chinese Pharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2009 Feb;34(3):275-8.
To optimize the formulation and preparation process of sinomenine liposomes.
Method of aether injection and mixture uniform design were adopted to determine the formulation of sinomenine liposomes is the proportion of phospholipids, cholesterol and Vitamin E with the index of entrapment efficiency. And the single-factor test was used to study the preparation process of the liposomes, including the volume of buffer solution, the preparation temperature and the ultrasonic time.
The optimized formulation was that the ratio of sinomenine : phospholipids : cholesterol : vitamin E mass ratio was 8.92 : 60.35 : 28.81 : 1.91. The volume of buffer solution was 50 mL x g(-1) membrane, the preparation temperature was 50 degrees C, and the ultrasonic time was 20 min.
Satisfactory shape and entrapment efficiency of the liposomes can be obtained by the optimized formulation and preparation process.
优化青藤碱脂质体的处方及制备工艺。
采用乙醚注入法和混合均匀设计，以包封率为指标确定青藤碱脂质体的处方，即磷脂、胆固醇和维生素E的比例。采用单因素试验研究脂质体制备工艺，包括缓冲液体积、制备温度和超声时间。
优化后的处方为青藤碱∶磷脂∶胆固醇∶维生素E质量比为8.92∶60.35∶28.81∶1.91。缓冲液体积为50 mL·g⁻¹膜，制备温度为50℃，超声时间为20 min。
通过优化的处方和制备工艺可获得形状和包封率均令人满意的脂质体。
