Zhang Dong-Qing, Cheng Yi, Bai Cong-Lin, Wu Qiong, Pang Gai-Hao
Department of Chinese Herbal Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China.
Zhong Yao Cai. 2011 May;34(5):786-9.
To prepare and characterize marine sterically stabilized liposomes (Marine-SSL).
Liposomes were prepared by ethanol injection technique. An orthogonal test was utilized to optimize the formulation and preparation of Marine-SSL The unencapsulated marine and liposomes were separated by sephadex gel G-50, the encapsulation efficiency was detected by HPLC. The morphological examination of Marine-SSL was performed using transmission electron microscopy. The particle size and Zeta potential of the liposomes were measured. The in vitro release rate of marine from liposomes was tested.
The liposomes with spherical or ellipsoidal shape and better stability featured the encapsulation efficiency of (85.39 +/- 1.21)%, the mean partical size of (156 +/- 10) nm, and Zeta potential of (- 39.0 +/- 3.06) mv. The release kinetics in vitro obeyed Higuchi equation. The stability of Marine-SSL was better.
The selected formulation and preparation technic of Marine-SSL are rational and stable and liposomes feature a sustained release in vitro.
制备并表征海洋空间稳定脂质体（Marine-SSL）。
采用乙醇注入法制备脂质体。利用正交试验优化Marine-SSL的处方和制备工艺。通过葡聚糖凝胶G-50分离未包封的海洋成分和脂质体，采用高效液相色谱法检测包封率。使用透射电子显微镜对Marine-SSL进行形态学检查。测定脂质体的粒径和Zeta电位。测试海洋成分从脂质体中的体外释放率。
脂质体呈球形或椭圆形，稳定性较好，包封率为（85.39±1.21）%，平均粒径为（156±10）nm，Zeta电位为（-39.0±3.06）mV。体外释放动力学符合Higuchi方程。Marine-SSL的稳定性较好。
所选用的Marine-SSL处方和制备工艺合理、稳定，脂质体具有体外缓释特性。
