Asai Shuta, Yoshioka Hirofumi
Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences. Nagoya University, Chikusa, Nagoya 464-8601, Japan.
Mol Plant Microbe Interact. 2009 Jun;22(6):619-29. doi: 10.1094/MPMI-22-6-0619.
Nitric oxide (NO) is an essential regulatory molecule in plant immunity in synergy with reactive oxygen species (ROS). However, little is known about the role of NO in disease resistance to necrotrophic pathogens. NO and oxidative bursts were induced during necrotrophic fungal pathogen Botrytis cinerea and Nicotiana benthamiana compatible interaction. Histochemical analyses showed that both NO and ROS were produced in adjacent cells of invaded areas in N. benthamiana leaves. Activation of salicylic acid-induced protein kinase, which regulates the radical burst, and several defense-related genes were induced after inoculation of B. cinerea. Loss-of-function analyses using inhibitors and virus-induced gene silencing were done to investigate the role of the radical burst in pathogenesis. We showed that NO plays a pivotal role in basal defense against B. cinerea and PR-1 gene expression in N. benthamiana. By contrast, ROS function has a negative role in resistance or has a positive role in expansion of disease lesions during B. cinerea-N. benthamiana interaction.
一氧化氮（NO）是植物免疫中与活性氧（ROS）协同作用的重要调节分子。然而，关于NO在对坏死营养型病原体的抗病性中的作用知之甚少。在坏死营养型真菌病原体灰葡萄孢与本氏烟草的亲和互作过程中，会诱导产生NO和氧化爆发。组织化学分析表明，在本氏烟草叶片被侵染区域的相邻细胞中会产生NO和ROS。接种灰葡萄孢后，水杨酸诱导的蛋白激酶（其调节自由基爆发）以及几个与防御相关的基因被激活。使用抑制剂和病毒诱导的基因沉默进行功能丧失分析，以研究自由基爆发在发病机制中的作用。我们表明，NO在本氏烟草对灰葡萄孢的基础防御和PR-1基因表达中起关键作用。相比之下，在灰葡萄孢与本氏烟草的互作过程中，ROS的功能在抗性中起负面作用，或在病害病斑扩展中起正面作用。
