Sutovská M, Nosálová G, Sutovský J, Franová S, Prisenznáková L, Capek P
Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Sklabinska 26, Martin, Slovakia.
Int J Biol Macromol. 2009 Jul 1;45(1):27-32. doi: 10.1016/j.ijbiomac.2009.03.008. Epub 2009 Apr 2.
The rhamnogalacturonan, isolated from the roots of medicinal plant Althaea officinalis L., showed various biological effects on the citric acid-induced cough reflex and reactivity of airways smooth muscle in vitro and in vivo conditions. It possessed dose-dependent cough suppression effect comparable with opioid agonist codeine. However, reactivity of the airways smooth muscle, measured in vitro as well as in vivo conditions was not significantly affected by rhamnogalacturonan and thus bronchodilatory activity did not participate in the cough suppression effect of polysaccharide tested. Moreover, the cough suppression effect of the polymer was not significantly modified by pretreatment of K(+)(ATP) ion channels with selective antagonist and therefore activation of this type of ion channels is not involved in the mechanism of rhamnogalacturonan cough suppressive ability. On the contrary, pretreatment of animals with selective 5-HT(2) receptors antagonist significantly decreased rhamnogalacturonan antitussive efficacy. From this point of view it seems that the cough suppression effect of the polymer is associated with the serotonergic 5-HT(2) receptor's function.
从药用植物药蜀葵（Althaea officinalis L.）根部分离得到的鼠李半乳糖醛酸聚糖，在体外和体内条件下，对柠檬酸诱导的咳嗽反射以及气道平滑肌反应性均表现出多种生物学效应。它具有与阿片类激动剂可待因相当的剂量依赖性镇咳作用。然而，在体外和体内条件下测定的气道平滑肌反应性并未受到鼠李半乳糖醛酸聚糖的显著影响，因此支气管扩张活性并未参与所测试多糖的镇咳作用。此外，用选择性拮抗剂预处理K(+)(ATP)离子通道后，聚合物的镇咳作用并未显著改变，因此这类离子通道的激活并不参与鼠李半乳糖醛酸聚糖的镇咳作用机制。相反，用选择性5-HT(2)受体拮抗剂预处理动物后，鼠李半乳糖醛酸聚糖的镇咳效果显著降低。从这一角度来看，该聚合物的镇咳作用似乎与5-羟色胺能5-HT(2)受体的功能有关。
