Alani Behrang, Zare Mohammad, Noureddini Mahdi
Department of Genetic and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Applied Cell Science, Kashan University of Medical Sciences, Kashan, Iran.
Department of Neuroscience Research Center, Isfahan University of Medical Sciences, Isfahan, Iran ; Department of Neurology, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2015 Mar 25;4:78. doi: 10.4103/2277-9175.153905. eCollection 2015.
The smooth muscle contractions of the tracheobronchial airways are mediated through the balance of adrenergic, cholinergic and peptidergic nervous mechanisms. This research was designed to determine the bronchodilatory and B-adrenergic effects of methanolic and aqueous extracts of root Althaea on the isolated tracheobronchial smooth muscle of the rat.
In this experimental study, 116 tracheobronchial sections (5 mm) from 58 healthy male Sprague-Dawley rats were dissected and divided into 23 groups. The effect of methanolic and aqueous extracts of the root Althaea was assayed at different concentrations (0.2, 0.6, 2.6, 6.6, 14.6 μg/ml) and epinephrine (5 μm) in the presence and absence of propranolol (1 μM) under one g tension based on the isometric method. This assay was recorded in an organ bath containing Krebs-Henseleit solution for tracheobronchial smooth muscle contractions using potassium chloride (KCl) (60 mM) induction.
Epinephrine (5 μm) alone and root methanolic and aqueous extract concentrations (0.6-14.6 μg/ml) reduced tracheobronchial smooth muscle contractions induced using KCl (60 mM) in a dose dependent manner. Propranolol inhibited the antispasmodic effect of epinephrine on tracheobronchial smooth muscle contractions, but could not reduce the antispasmodic effect of the root extract concentrations.
The methanolic and aqueous extracts of Althaea root inhibited the tracheobronchial smooth muscle contractions of rats in a dose dependent manner, but B-adrenergic receptors do not appear to engage in this process. Understanding the mechanism of this process can be useful in the treatment of pulmonary obstructive diseases like asthma.
气管支气管气道的平滑肌收缩是通过肾上腺素能、胆碱能和肽能神经机制的平衡来介导的。本研究旨在确定药蜀葵根的甲醇提取物和水提取物对大鼠离体气管支气管平滑肌的支气管扩张作用和β-肾上腺素能作用。
在本实验研究中，从58只健康雄性Sprague-Dawley大鼠身上解剖出116个气管支气管节段（5毫米），并分为23组。根据等长法，在1克张力下，在有无普萘洛尔（1μM）的情况下，测定药蜀葵根的甲醇提取物和水提取物在不同浓度（0.2、0.6、2.6、6.6、14.6μg/ml）以及肾上腺素（5μm）对气管支气管平滑肌收缩的影响。该测定在含有用于气管支气管平滑肌收缩的Krebs-Henseleit溶液的器官浴中进行，使用氯化钾（KCl）（60mM）诱导。
单独使用肾上腺素（5μm）以及根甲醇提取物和水提取物浓度（0.6 - 14.6μg/ml）均以剂量依赖方式降低了使用KCl（60mM）诱导的气管支气管平滑肌收缩。普萘洛尔抑制了肾上腺素对气管支气管平滑肌收缩的解痉作用，但不能降低根提取物浓度的解痉作用。
药蜀葵根的甲醇提取物和水提取物以剂量依赖方式抑制大鼠气管支气管平滑肌收缩，但β-肾上腺素能受体似乎未参与此过程。了解这一过程的机制可能有助于治疗哮喘等肺部阻塞性疾病。
