姜黄素通过抑制 MMP-9 的表达来防止人主动脉平滑肌细胞的迁移。
Curcumin prevents human aortic smooth muscle cells migration by inhibiting of MMP-9 expression.
机构信息
Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan, Taiwan.
出版信息
Nutr Metab Cardiovasc Dis. 2010 Feb;20(2):125-32. doi: 10.1016/j.numecd.2009.03.001. Epub 2009 May 17.
BACKGROUND AND AIM
The migration of vascular smooth muscle cells from the tunica media to the subendothelial region is a key event in the development of atherosclerosis. Curcumin, which is consumed daily by millions of people, is a polyphenol derived from the plant Curcuma longa. In this study, we investigated the effects of curcumin on tumor necrosis factor-alpha (TNF-alpha)-induced cell migration, the formation of intracellular reactive oxygen species (ROS), the translocation of nuclear factor-kappaB (NFkappaB) and the activation and expression of MMP-9 in human aortic smooth muscle cells (HASMCs).
METHODS AND RESULTS
The Matrigel migration assay showed that curcumin (10 and 20 micromol/l) effectively inhibited TNF-alpha-induced migration of HASMCs as compared with the control group. To explain this inhibitory effect, MMP-9 was assayed by gelatin zymography and Western blot. The results indicated that curcumin inhibited MMP-9 activity and expression. Furthermore, the production of ROS and the nuclear translocation of NF-kappaB p50 and p65 induced by TNF-alpha were dose-dependently suppressed by curcumin pretreatment.
CONCLUSION
These results indicate that curcumin has anti-inflammatory properties and may prevent the migration of HASMCs by suppressing MMP-9 expression through down-regulation of NF-kappaB.
背景与目的
血管平滑肌细胞从中膜迁移到内膜下区域是动脉粥样硬化发展的关键事件。姜黄素是从植物姜黄中提取的多酚，每天都有数百万人食用。本研究旨在探讨姜黄素对肿瘤坏死因子-α（TNF-α）诱导的人主动脉平滑肌细胞（HASMC）迁移、细胞内活性氧（ROS）形成、核因子-κB（NF-κB）易位以及 MMP-9 激活和表达的影响。
方法与结果
Matrigel 迁移实验表明，与对照组相比，姜黄素（10 和 20 μmol/L）可有效抑制 TNF-α诱导的 HASMC 迁移。为了解释这种抑制作用，通过明胶酶谱法和 Western blot 检测 MMP-9。结果表明，姜黄素抑制 MMP-9 活性和表达。此外，姜黄素预处理可剂量依赖性抑制 TNF-α诱导的 ROS 产生和 NF-κB p50 和 p65 的核易位。
结论
这些结果表明，姜黄素具有抗炎特性，可能通过下调 NF-κB 抑制 MMP-9 表达来防止 HASMC 迁移。
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