Villa R F, Turpeenoja L, Magrì G, Gorini A, Ragusa N, Giuffrida-Stella A M
Institute of Pharmacology, Faculty of Science, University of Pavia, Italy.
Neurochem Res. 1991 Jul;16(7):821-6. doi: 10.1007/BF00965692.
The effect of hypoxia on the protein composition of mitochondria from cerebral cortex of rats at 4, 12, and 24 months of age was investigated. The proteins were separated by electrophoresis on SDS polyacrylamide gels and the percent content was evaluated by measuring the optical density of the stained gels. The results demonstrate that hypoxic treatment causes a decrease in the amount of some proteins as follows: the 90 and the 16 kDa Mw proteins at 4 months; the 82 and the 79 kDa Mw proteins at 24 months; the 52-49, 35 and 20 kDa at all ages investigated; the 44 kDa protein at 4 and 12 months and the 28 kDa protein at 4 and 24 months of age. Our results show that hypoxic conditions affect mitochondrial protein composition to a greater extent than aging alone.
研究了缺氧对4、12和24月龄大鼠大脑皮质线粒体蛋白质组成的影响。蛋白质通过SDS聚丙烯酰胺凝胶电泳分离，通过测量染色凝胶的光密度评估其百分比含量。结果表明，缺氧处理导致某些蛋白质的含量降低，具体如下：4月龄时分子量为90和16 kDa的蛋白质；24月龄时分子量为82和79 kDa的蛋白质；在所有研究年龄中分子量为52 - 49、35和20 kDa的蛋白质；4和12月龄时分子量为44 kDa的蛋白质以及4和24月龄时分子量为28 kDa的蛋白质。我们的结果表明，缺氧条件对线粒体蛋白质组成的影响比单独衰老更大。
