Benzi G, Arrigoni E, Pastoris O, Villa R F, Dossena M, Agnoli A, Giuffrida A M
J Cereb Blood Flow Metab. 1982;2(2):229-39. doi: 10.1038/jcbfm.1982.23.
The synaptosomal fractions obtained from the motor area of the cerebral cortex of normocapnic, normoxic, or hypoxic, untreated beagle dogs and of pentobarbital (Nembutal)- or cytidine diphosphate (CDP)-choline-treated dogs were incubated and analyzed for ATP, ADP, AMP, creatine phosphate, pyruvate, and lactate. The data were compared with data obtained by the surface freezing technique from the whole contralateral cortical area. The in vivo intracarotid perfusion of the drug differentially affected the content of the metabolites and their ratio. This occurred whether the evaluations were performed in the incubated synaptosomal preparations or in whole cerebral tissue, both during normoxia and after hypoxia (15 min; PaO2 = 17-19 mm Hg). Thus, intracarotid perfusion of nembutal increased the synaptosomal phosphorylation state both in normoxic and in hypoxic animals, whereas the effect on the metabolism of the contralateral cortical motor area as a whole was in all cases less than that observed in the synaptosomal fraction. Perfusion with CDP-choline increased synaptosomal phosphorylation after the hypoxic condition, but had no effect in normoxia or on the whole cortical tissue of the motor area. The possibility of obtaining a cerebral sparing action by utilizing molecules devoid of anesthetic action is suggested.
从正常碳酸血症、常氧或低氧、未经处理的比格犬以及经戊巴比妥（戊巴比妥钠）或胞苷二磷酸（CDP）-胆碱处理的犬的大脑皮质运动区获得的突触体组分进行孵育，并分析其中的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）、磷酸肌酸、丙酮酸和乳酸。将这些数据与通过表面冷冻技术从对侧整个皮质区域获得的数据进行比较。药物的体内颈内灌注对代谢物的含量及其比率有不同影响。无论评估是在孵育的突触体制剂中还是在全脑组织中进行，在常氧期间和低氧（15分钟；动脉血氧分压=17-19毫米汞柱）后均会出现这种情况。因此，戊巴比妥的颈内灌注增加了常氧和低氧动物突触体的磷酸化状态，而对整个对侧皮质运动区代谢的影响在所有情况下均小于在突触体组分中观察到的影响。CDP-胆碱灌注在低氧条件后增加了突触体磷酸化，但在常氧时或对运动区的整个皮质组织没有影响。这提示了利用无麻醉作用的分子获得脑保护作用的可能性。
