Norenberg M D, Baker L, Norenberg L O, Blicharska J, Bruce-Gregorios J H, Neary J T
Laboratory of Neuropathology, Veterans Administration Medical center, Miami, FL.
Neurochem Res. 1991 Jul;16(7):833-6. doi: 10.1007/BF00965694.
The effect of ammonia on water space of astrocytes in culture was determined as a means of studying the neurotoxicity of ammonia in fulminant hepatic failure (FHF). Treatment of primary astrocyte cultures obtained from neonatal rat cortices with 10 mM NH4Cl for 4 days resulted in a 29% increase in astrocytic water space, as measured by an isotopic method utilizing 3-O-methyl-[3H]-glucose. This effect was time- and dose-dependent. The ammonia-induced swelling was reversible as the water space in cultures treated with 10 mH NH4Cl for 3 days, and then returned to normal culture media for 1 day, was similar to control cultures. These findings suggest that elevated levels of ammonia lead to astrocyte swelling and may contribute to the brain edema in FHF.
作为研究暴发性肝衰竭（FHF）中氨的神经毒性的一种手段，测定了氨对培养的星形胶质细胞水空间的影响。用10 mM氯化铵处理从新生大鼠皮质获得的原代星形胶质细胞培养物4天，通过利用3-O-甲基-[3H]-葡萄糖的同位素方法测量，星形胶质细胞水空间增加了29%。这种效应具有时间和剂量依赖性。在用10 mH氯化铵处理3天然后返回正常培养基1天的培养物中，水空间与对照培养物相似，这表明氨诱导的肿胀是可逆的。这些发现表明，氨水平升高会导致星形胶质细胞肿胀，并可能导致FHF中的脑水肿。
