Mattern C F, Keister D B
Am J Trop Med Hyg. 1977 May;26(3):393-401.
Axenically cultured Entamoeba histolytica inoculated intracerebrally into newborn Swiss mice invaded the cerebrum and produced multiple abscesses containing viable trophozoites. As few as 20 amebae of a virulent strain (HM-1:IMSS) occasionally produced fatal disease, 200 killed about 75% of animals and higher doses regularly killed all animals. In contrast, avirulent strains (HK-9 and HB-301:NIH) failed to produce acute brain disease in comparable time periods even when mice were inoculated with as many as 20,000 amebae. Two other strains (1295 and H-458:CDC) were of intermediate virulence. High doses of avirulent amebae often produced hydrocephalus as a late manifestation. In newborn, 3 week-old, and 6-week-old mice resistance to infection increased with age, and older animals often responded late to virulent strains by developing hydrocephalus.
将无菌培养的溶组织内阿米巴脑内接种到新生瑞士小鼠体内后，它会侵入大脑并产生多个含有活滋养体的脓肿。毒力强的菌株（HM-1:IMSS），少至20个阿米巴偶尔会导致致命疾病，200个会杀死约75%的动物，更高剂量则会定期杀死所有动物。相比之下，无毒力的菌株（HK-9和HB-301:NIH）即使给小鼠接种多达20000个阿米巴，在相当长的时间段内也不会引发急性脑病。另外两个菌株（1295和H-458:CDC）的毒力处于中等水平。高剂量的无毒力阿米巴常常会在后期引发脑积水。在新生小鼠、3周龄小鼠和6周龄小鼠中，对感染的抵抗力随年龄增长而增强，年龄较大的动物对毒力强的菌株的反应往往较晚，表现为出现脑积水。
