Mattern C F, Keister D B
Am J Trop Med Hyg. 1977 May;26(3):402-11.
Newborn hamsters inoculated intrahepatically were highly susceptible to infection by axenically cultured Entamoeba histolytica. Inoculations were performed through the abdominal wall, and lesions could be observed through the skin as early as 4 days after inoculation. The most virulent amebal strain, HM-1:IMSS, produced liver lesions in 19% of newborn animals inoculated with 20 amebae, and in about 90% receiving 2,000 amebae. Eleven other strains similarly tested either produced no lesions with 20,000 amebae or were of intermediate virulence. Two hamster strains did not show appreciable differences in susceptibility to the HM-1:IMSS amebal strain. Newborn hamsters were more susceptible to HM-1:IMSS amebae than animals which were 2, 4, or 7 days old at the time of inoculation. Three-week-old animals were resistant to doses below 20,000 virulent amebae.
经肝内接种的新生仓鼠对无菌培养的溶组织内阿米巴感染高度敏感。接种是通过腹壁进行的，最早在接种后4天就能透过皮肤观察到病变。毒性最强的阿米巴菌株HM-1:IMSS，在接种20个阿米巴的新生动物中有19%出现肝脏病变，在接种2000个阿米巴的动物中约90%出现病变。同样测试的其他11个菌株，接种20000个阿米巴时要么不产生病变，要么具有中等毒力。两种仓鼠品系对HM-1:IMSS阿米巴菌株的易感性没有明显差异。新生仓鼠比接种时2、4或7日龄的动物对HM-1:IMSS阿米巴更敏感。3周龄的动物对低于20000个有毒力阿米巴的剂量具有抗性。
