Mattern C F, Keister D B, Caspar P A
Am J Trop Med Hyg. 1978 Sep;27(5):882-7.
A rapid and simple assay for cytopathogenicity of axenically cultivated Entamoeba histolytica has been developed employing baby hamster kidney (BHK) or mouse 3T3 cells conventionally tissue cultured. Three of the twelve amebal strains tested produced total destruction of the BHK cell monolayer in 2--3 hours, and these three strains are the three most virulent strains for the newborn hamster liver. Two additional strains were of moderate cytopathogenicity in vitro and of moderate virulence in vivo. Seven strains were of low cytopathogenicity and virulence. Within these three major groupings, however, the cytopathogenicity ranking was not entirely reproducible. The general correspondence of cytopathogenicity in vitro and virulence in vivo and the possibility of obtaining data within a few hours suggest such an assay may prove a useful tool in amebiasis research.
已开发出一种快速简便的检测方法，用于检测无菌培养的溶组织内阿米巴的细胞致病性，该方法采用常规组织培养的幼仓鼠肾（BHK）细胞或小鼠3T3细胞。在测试的12个阿米巴菌株中，有3个菌株在2至3小时内使BHK细胞单层完全破坏，这3个菌株是对新生仓鼠肝脏毒性最强的3个菌株。另外两个菌株在体外具有中等细胞致病性，在体内具有中等毒力。7个菌株的细胞致病性和毒力较低。然而，在这三个主要类别中，细胞致病性排名并不完全可重复。体外细胞致病性与体内毒力的总体对应关系以及在几小时内获得数据的可能性表明，这种检测方法可能被证明是阿米巴病研究中的一种有用工具。
