Smith E J
Avian Dis. 1977 Apr-Jun;21(2):290-9.
Immunization of rabbits with a pooled preparation of chromato-graphically purified avian myeloblastosis virus (AMV) group-specific (gs) antigens produced relatively large volumes of antiserum that was as broadly reactive in complement-fixation (CF) tests as antiserum produced in hamsters with tumors induced by Rous sarcoma virus. This alternate procedure should be of value for routine preparation of leukosis virus gs antiserum. Other antisera prepared against disrupted AMV had spurious reactivities that confused CF testing of embryos and tissue extracts. This artifact was attributed to the high affinity of serum albumin for AMV virions and the resultant production of antibodies when plasma-derived AMV was used as immunogen. A high-molecular-weight AMV CF antigen was also found in early fractions eluted from an agarose-gel column.
用经色谱纯化的禽成髓细胞瘤病毒（AMV）群特异性（gs）抗原的混合制剂免疫兔子，可产生相对大量的抗血清，该抗血清在补体结合（CF）试验中的反应范围与用劳斯肉瘤病毒诱导的肿瘤免疫仓鼠所产生的抗血清一样广泛。这种替代方法对于白血病病毒gs抗血清的常规制备应该是有价值的。针对裂解的AMV制备的其他抗血清具有虚假反应性，这使得胚胎和组织提取物的CF检测变得混乱。这种假象归因于血清白蛋白对AMV病毒粒子的高亲和力，以及当血浆来源的AMV用作免疫原时产生的抗体。在从琼脂糖凝胶柱洗脱的早期组分中还发现了一种高分子量的AMV CF抗原。
