活化的巨噬细胞诱导结肠癌细胞的转移行为。

Activated macrophages induce metastatic behavior of colon cancer cells.

机构信息

Cancer Research Laboratory, Methodist Research Institute, 1800 N Capitol Ave, E504, Indianapolis, IN 46202, USA.

出版信息

Immunobiology. 2010 Mar;215(3):242-9. doi: 10.1016/j.imbio.2009.03.004. Epub 2009 May 19.

DOI:10.1016/j.imbio.2009.03.004

PMID:19457576

Abstract

Tumor-associated macrophages were linked to the growth, angiogenesis, and metastasis of variety of cancers. However, the role of macrophages in colon cancer is elusive. In the present study, we demonstrate that activated macrophage-conditioned medium (AMCM), containing tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6, markedly induced proliferation and migration of human colon cancer cells HCT116. Furthermore, AMCM significantly increased activation of transcription factor NF-kappaB and secretion of vascular endothelial growth factor (VEGF) from colon cancer cells, which subsequently induced capillary morphogenesis of human aortic endothelial cells. In conclusion, the interruption of signaling between activated macrophages and colon cancer cells could be considered as a new therapeutic strategy.

摘要

肿瘤相关巨噬细胞与多种癌症的生长、血管生成和转移有关。然而，巨噬细胞在结肠癌中的作用尚不清楚。在本研究中，我们证明了含有肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和 IL-6 的活化巨噬细胞条件培养基（AMCM）可显著诱导人结肠癌细胞 HCT116 的增殖和迁移。此外，AMCM 还显著增加了转录因子 NF-κB 的激活和血管内皮生长因子（VEGF）的分泌，从而诱导人主动脉内皮细胞的毛细血管形态发生。总之，阻断活化巨噬细胞与结肠癌细胞之间的信号传递可以被视为一种新的治疗策略。