通过通用聚合酶链反应结合高分辨率熔解分析快速鉴定生物威胁及其他临床相关细菌种类。
Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis.
作者信息
Yang Samuel, Ramachandran Padmini, Rothman Richard, Hsieh Yu-Hsiang, Hardick Andrew, Won Helen, Kecojevic Aleksandar, Jackman Joany, Gaydos Charlotte
机构信息
Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD 21209, USA.
出版信息
J Clin Microbiol. 2009 Jul;47(7):2252-5. doi: 10.1128/JCM.00033-09. Epub 2009 May 20.
Abstract
A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.
摘要
本文描述了一种用于真细菌物种鉴定的快速检测方法，该方法利用高分辨率熔解分析来表征PCR产物。从100种临床相关细菌病原体的16S rRNA基因的多个高变区生成的独特熔解曲线，包括A类和B类生物威胁因子及其替代物，能够实现高度特异性的物种鉴定。
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