Morgenstern J P, Griffith I J, Brauer A W, Rogers B L, Bond J F, Chapman M D, Kuo M C
ImmuLogic Pharmaceutical Corp., Cambridge, MA 02139.
Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9690-4. doi: 10.1073/pnas.88.21.9690.
The complete primary structure of Fel dI (International Union of Immunological Societies nomenclature), the major allergen produced by the domestic cat, Felis domesticus, was determined by protein sequence analysis and cDNA cloning. Protein sequencing of Fel dI from an immunoaffinity-purified extract of house dust revealed that the allergen is composed of two polypeptide chains. Degenerate oligonucleotides derived from the protein sequence were used in polymerase chain reaction amplification of cat salivary gland cDNA to demonstrate that the two chains are encoded by different genes. Chain 1 of Fel dI shares amino acid homology with rabbit uteroglobin, while chain 2 is a glycoprotein with N-linked oligosaccharides.
家猫（Felis domesticus）产生的主要过敏原Fel dI（国际免疫学会命名法）的完整一级结构通过蛋白质序列分析和cDNA克隆得以确定。对来自室内灰尘免疫亲和纯化提取物的Fel dI进行蛋白质测序表明，该过敏原由两条多肽链组成。从蛋白质序列推导而来的简并寡核苷酸用于猫唾液腺cDNA的聚合酶链反应扩增，以证明这两条链由不同基因编码。Fel dI的链1与兔子宫球蛋白具有氨基酸同源性，而链2是一种带有N-连接寡糖的糖蛋白。
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