用PAA修饰的Fe3O4@二氧化硅核壳微球对溶菌酶进行有效吸附和分离。
Effective adsorption and separation of lysozyme with PAA-modified Fe3O4@silica core/shell microspheres.
作者信息
Shao Dandan, Xu Keke, Song Xiaojie, Hu Jianhua, Yang Wuli, Wang Changchun
机构信息
Key Laboratory of Molecular Engineering of Polymers (Ministry of Education), Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
出版信息
J Colloid Interface Sci. 2009 Aug 15;336(2):526-32. doi: 10.1016/j.jcis.2009.02.061. Epub 2009 Apr 8.
Submicron-size Fe(3)O(4)@silica core/shell microspheres were prepared by sol-gel method. Through coating inert silica shell and adjusting the thickness, the dispersibility of magnetic microspheres in water was greatly improved. The resultant Fe(3)O(4)@silica microspheres with controllable saturation magnetization between 34.3 emu/g and 47.3 emu/g were superparamagnetic. All as-prepared magnetic composite microspheres possessed fleetly magnetic responsivity. The PAA-modified Fe(3)O(4)@silica microspheres were used for selective adsorption and separation of lysozyme, and the experimental results showed that the maximum binding capacity is about 127 mg/g. After surface modification of Fe(3)O(4)@silica with polyacrylic acid (PAA) flexible chains, the binding capacity of Fe(3)O(4)@silica-g-PAA microspheres for lysozyme were 22 times as much as that of pure Fe(3)O(4)@silica microspheres at the same pH condition.
通过溶胶-凝胶法制备了亚微米级的Fe(3)O(4)@二氧化硅核壳微球。通过包覆惰性二氧化硅壳层并调节其厚度，磁性微球在水中的分散性得到了极大改善。所得的Fe(3)O(4)@二氧化硅微球的饱和磁化强度在34.3 emu/g至47.3 emu/g之间可控，具有超顺磁性。所有制备的磁性复合微球都具有快速的磁响应性。用聚丙烯酸（PAA）修饰的Fe(3)O(4)@二氧化硅微球用于溶菌酶的选择性吸附和分离，实验结果表明最大结合容量约为127 mg/g。在用聚丙烯酸（PAA）柔性链对Fe(3)O(4)@二氧化硅进行表面修饰后，在相同pH条件下，Fe(3)O(4)@二氧化硅-g-PAA微球对溶菌酶的结合容量是纯Fe(3)O(4)@二氧化硅微球的22倍。
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