Martínez Chamás José, Isla María Inés, Zampini Iris Catiana
Laboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Bioprospección y Fisiología Vegetal (INBIOFIV-CONICET-UNT), Facultad de Ciencias Naturales e IML, Universidad Nacional de Tucumán, San Lorenzo 1469, San Miguel de Tucumán PC:4000, Tucumán, Argentina.
Plants (Basel). 2023 Apr 29;12(9):1830. doi: 10.3390/plants12091830.
is an opportunistic pathogen that, under certain conditions, can induce aggravated infectious processes, mainly in immunosuppressed patients. Moreover, is one of the leading causes of medical device- and implant-associated infections and is also recognized as a canonical biofilm producer. , and are three medicinal plants that grow in arid environments in Argentina (Altoandina, Puna, Prepuna and Monte regions). In this work, we studied the antimicrobial activity of alcoholic extracts of these plant species obtained via maceration (M) and ultrasound-assisted extraction (UAE) against . In addition, the antibiofilm activity of the extract was also evaluated. It was found that the extracts obtained via M did not present differences with those obtained via UAE regarding the chemical profile. showed the lowest minimum inhibitory concentration (MIC) value (75 µg GAE/mL). At concentrations higher than the MIC, the extract induced the release of cellular constituents. At the concentration of 1/8× MIC, the extract inhibited biofilm formation by 78%, reducing metabolic activity by 67%. On the other hand, it presented a low percentage of preformed biofilm removal. In all assays, gallic acid (GA) has been used as a reference antimicrobial compound. Finally, it was shown via microscopy visualization that the extract reduces adhesion to hydrophobic and hydrophilic surfaces. Thus, extracts could potentially be used for the antibiotic treatment of infections produced by or as an inhibitor agent of production biofilm, avoiding infections caused by medical devices.
是一种机会致病菌，在某些条件下，主要在免疫抑制患者中可引发严重感染过程。此外，它是医疗器械和植入物相关感染的主要原因之一，也被认为是典型的生物膜产生菌。[植物名称1]、[植物名称2]和[植物名称3]是生长在阿根廷干旱环境（阿尔托安第纳、普纳、前普纳和蒙特地区）的三种药用植物。在这项工作中，我们研究了通过浸渍法（M）和超声辅助提取法（UAE）获得的这些植物物种的醇提取物对[致病菌名称]的抗菌活性。此外，还评估了[植物名称]提取物的抗生物膜活性。发现通过浸渍法获得的提取物与通过超声辅助提取法获得的提取物在化学特征方面没有差异。[植物名称]显示出最低的最低抑菌浓度（MIC）值（75μg没食子酸当量/mL）。在高于MIC的浓度下，该提取物诱导细胞成分的释放。在1/8×MIC浓度下，该提取物抑制生物膜形成达78%，使代谢活性降低67%。另一方面，它对已形成的生物膜的去除率较低。在所有试验中，没食子酸（GA）被用作参考抗菌化合物。最后，通过显微镜观察表明，该提取物减少了对疏水和亲水表面的粘附。因此，[植物名称]提取物有可能用于[致病菌名称]引起的感染的抗生素治疗，或作为生物膜产生的抑制剂，避免医疗器械引起的感染。
