Malaisse Willy J, Louchami Karim, Sener Abdullah
Université Libre de Bruxelles, Brussels, Belgium.
Nat Rev Endocrinol. 2009 Jul;5(7):394-400. doi: 10.1038/nrendo.2009.103. Epub 2009 May 26.
Noninvasive imaging and quantification of pancreatic, insulin-producing beta cells has been considered a high-priority field of investigation for the past decade. In the first review on this issue, attention was already paid to various agents for labeling beta cells, including 6-(125)I-D-glucose, (65)Zn, (3)H-glibenclamide, (3)H-mitiglinide, an (125)I-labeled mouse monoclonal antibody against beta-cell surface ganglioside(s), D-(U-(14)C)-glucose and 2-deoxy-2-(18)F-D-glucose to label glycogen accumulated in beta cells in response to sustained hyperglycemia, and, last but not least, an analog of D-mannoheptulose. This Review discusses these methods and further contributions. For instance, emphasis is placed on labeling beta cells with (11)C-dihydrotetrabenazine, which is the most advanced method at present. Attention is also drawn to the latest approaches for noninvasive imaging and functional characterization of pancreatic beta cells. None of these procedures is used in clinical practice yet.
在过去十年中，对胰腺中产生胰岛素的β细胞进行无创成像和定量分析一直被视为一个高度优先的研究领域。在关于这个问题的第一篇综述中，就已经关注到了各种用于标记β细胞的试剂，包括6-(125)I-D-葡萄糖、(65)Zn、(3)H-格列本脲、(3)H-米格列奈、一种针对β细胞表面神经节苷脂的(125)I标记小鼠单克隆抗体、D-(U-(14)C)-葡萄糖和2-脱氧-2-(18)F-D-葡萄糖，用于标记β细胞因持续性高血糖而积累的糖原，最后但同样重要的是，D-甘露庚酮糖的类似物。本综述讨论了这些方法以及进一步的贡献。例如，重点介绍了用(11)C-二氢四苯嗪标记β细胞的方法，这是目前最先进的方法。还关注了胰腺β细胞无创成像和功能表征的最新方法。这些方法目前均未应用于临床实践。
