Yao Xin-Cheng, Cui Wan-Xing, Li Yi-Chao, Zhang Wei, Lu Rong-Wen, Thompson Anthony, Amthor Franklin, Wang Xu-Jing
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
J Mod Opt. 2012 May 1;59(9). doi: 10.1080/09500340.2012.674564.
We demonstrate intrinsic optical signal (IOS) imaging of intact rat islet, which consists of many endocrine cells working together. A near-infrared digital microscope was employed for optical monitoring of islet activities evoked by glucose stimulation. Dynamic NIR images revealed transient IOS responses in the islet activated by low-dose (2.75mM) and high-dose (5.5mM) glucose stimuli. Comparative experiments and quantitative analysis indicated that both glucose metabolism and calcium/insulin dynamics might contribute to the observed IOS responses. Further investigation of the IOS imaging technology may provide a high resolution method for functional examination of the islet, which is important for advanced study of diabetes associated islet dysfunctions and for improved quality control of donor islets for transplantation.
我们展示了完整大鼠胰岛的内在光学信号（IOS）成像，胰岛由许多共同发挥作用的内分泌细胞组成。使用近红外数字显微镜对葡萄糖刺激诱发的胰岛活动进行光学监测。动态近红外图像显示，低剂量（2.75mM）和高剂量（5.5mM）葡萄糖刺激激活的胰岛中出现了短暂的IOS反应。对比实验和定量分析表明，葡萄糖代谢以及钙/胰岛素动态变化可能都对观察到的IOS反应有贡献。对IOS成像技术的进一步研究可能会为胰岛的功能检测提供一种高分辨率方法，这对于糖尿病相关胰岛功能障碍的深入研究以及改善移植供体胰岛的质量控制非常重要。
