P25-Biomedical Spectroscopy, Robert Koch Institute, Nordufer 20, D-13353 Berlin, Germany.
Analyst. 2009 Jun;134(6):1149-53. doi: 10.1039/b822574e. Epub 2009 Mar 25.
Single cells in genetically homogeneous microbial cultures exhibit marked phenotypic heterogeneity that is considered to bolster the fitness of the whole population. Heterogeneity on the single-cell level is typically masked in conventional studies of microbial populations, which rely on data averaged across thousands or millions of cells in a sample. Here we introduce confocal Raman microspectroscopy as a method for investigating and illustrating the spatial heterogeneity of microbial cell populations. By the use of three different test organisms as model systems, we show pronounced cellular heterogeneity even in colonies cultivated under laboratory conditions.
在遗传上同质的微生物培养物中的单个细胞表现出明显的表型异质性，这被认为可以增强整个种群的适应性。在依赖于对样本中数千或数百万个细胞进行平均的数据的传统微生物种群研究中，通常会掩盖单细胞水平上的异质性。在这里，我们介绍共焦拉曼微光谱作为一种研究和说明微生物细胞群体空间异质性的方法。通过使用三种不同的测试生物作为模型系统，我们甚至在实验室条件下培养的菌落中也显示出明显的细胞异质性。
