Key Laboratory of Biorheological Science and Technology of Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Colloids Surf B Biointerfaces. 2012 Jun 1;94:347-53. doi: 10.1016/j.colsurfb.2012.02.009. Epub 2012 Feb 16.
To improve surface endothelialization of NiTi alloy substrate, a nano-structured coating functionalized with vascular endothelial growth factor (VEGF) was fabricated via polydopamine (PDOP) as intermediate layer. The successful preparation of VEGF conjugated nanocoating was demonstrated by X-ray diffraction (XRD), atomic force microscope (AFM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS), respectively. Inductively coupled plasma mass spectrometry (ICP-MS) test showed that the formed nanocoating significantly reduced the release of Ni ion from NiTi alloy in simulated body fluid. The biological behaviors of endothelial cells adhered to modified NiTi alloy substrates, including cell proliferation, cell spreading and production of nitric oxide and prostacyclin were investigated in vitro. The results suggest that surface functionalization of NiTi alloy substrate with VEGF is beneficial for cell growth. The approach presented here affords an alternative for surface modification of NiTi implants applied as heart and vascular implant devices.
为改善 NiTi 合金基底的表面内皮化，通过聚多巴胺（PDOP）作为中间层，制备了一种具有血管内皮生长因子（VEGF）功能的纳米结构涂层。X 射线衍射（XRD）、原子力显微镜（AFM）、扫描电子显微镜（SEM）和 X 射线光电子能谱（XPS）分别证明了 VEGF 接枝纳米涂层的成功制备。电感耦合等离子体质谱（ICP-MS）测试表明，形成的纳米涂层显著减少了 NiTi 合金在模拟体液中 Ni 离子的释放。体外研究了内皮细胞在改性 NiTi 合金基底上的黏附、增殖、铺展以及一氧化氮和前列环素的生成等生物学行为。结果表明，VEGF 对 NiTi 合金基底的表面功能化有利于细胞生长。该方法为心脏和血管植入器件等 NiTi 植入物的表面改性提供了一种替代方法。
