Heo Soo-Jin, Hwang Ji-Young, Choi Jung-In, Han Ji-Sook, Kim Hak-Ju, Jeon You-Jin
Marine Living Resources Research Department, Korea Ocean Research & Development Institute, Ansan, 426-744, South Korea.
Eur J Pharmacol. 2009 Aug 1;615(1-3):252-6. doi: 10.1016/j.ejphar.2009.05.017. Epub 2009 May 29.
This study was designed to investigate whether diphlorethohydroxycarmalol (DPHC) may inhibit alpha-glucosidase and alpha-amylase activities, and alleviate postprandial hyperglycemia in streptozotocin-induced diabetic mice. DPHC isolated from Ishige okamurae, a brown algae, evidenced prominent inhibitory effect against alpha-glucosidase and alpha-amylase. The IC(50) values of DPHC against alpha-glucosidase and alpha-amylase were 0.16 and 0.53 mM, respectively, which evidenced the higher activities than that of acarbose. DPHC did not exert any cytotoxic effect in human umbilical vein endothelial cells (HUVECs) at various concentrations (from 0.49 to 3.91 mM). The increase of postprandial blood glucose levels were significantly suppressed in the DPHC-administered group than those in the streptozotocin-induced diabetic or normal mice. Moreover, the area under curve (AUC) was significantly reduced via DPHC administration (2022 versus 2210 mmol x min/l) in the diabetic mice as well as it delays absorption of dietary carbohydrates. Therefore, these result indicated that DPHC might be a potent inhibitor for alpha-glucosidase and alpha-amylase.
本研究旨在探讨二氢褐藻醇（DPHC）是否能抑制α-葡萄糖苷酶和α-淀粉酶的活性，并减轻链脲佐菌素诱导的糖尿病小鼠的餐后高血糖。从褐藻冈村石莼中分离得到的DPHC对α-葡萄糖苷酶和α-淀粉酶具有显著的抑制作用。DPHC对α-葡萄糖苷酶和α-淀粉酶的IC50值分别为0.16和0.53 mM，这表明其活性高于阿卡波糖。在不同浓度（0.49至3.91 mM）下，DPHC对人脐静脉内皮细胞（HUVECs）没有任何细胞毒性作用。与链脲佐菌素诱导的糖尿病小鼠或正常小鼠相比，给予DPHC的组餐后血糖水平的升高得到了显著抑制。此外，通过给予DPHC，糖尿病小鼠的曲线下面积（AUC）显著降低（2022对2210 mmol·min/l），并且它还延迟了膳食碳水化合物的吸收。因此，这些结果表明DPHC可能是一种有效的α-葡萄糖苷酶和α-淀粉酶抑制剂。
