Department of Marine life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Food Chem Toxicol. 2010 Oct;48(10):2633-7. doi: 10.1016/j.fct.2010.06.032. Epub 2010 Jun 25.
This study was designed to investigate whether dieckol may inhibit α-glucosidase and alpha-amylase activities, and alleviate postprandial hyperglycemia in streptozotocin-induced diabetic mice. Dieckol isolated from Ecklonia cava, brown algae, evidenced prominent inhibitory effect against alpha-glucosidase and alpha-amylase. The IC(50) values of dieckol against alpha-glucosidase and alpha-amylase were 0.24 and 0.66 mM, respectively, which evidenced the higher activities than that of acarbose. Dieckol did not exert any cytotoxic effect in human umbilical vein endothelial cells (HUVECs) at various concentrations (from 0.33 to 2.69 mM). The increase of postprandial blood glucose levels were significantly suppressed in the dieckol administered group than those in the streptozotocin-induced diabetic or normal mice. Moreover, the area under curve (AUC) was significantly reduced via dieckol administration (259 versus 483 mmol min/l) in the diabetic mice as well as it delays absorption of dietary carbohydrates. Therefore, these result indicated that dieckol might be a potent inhibitor for α-glucosidase and α-amylase.
本研究旨在探讨藻褐素是否可以抑制α-葡萄糖苷酶和α-淀粉酶的活性，以及减轻链脲佐菌素诱导的糖尿病小鼠的餐后高血糖。从褐藻中分离得到的藻褐素对α-葡萄糖苷酶和α-淀粉酶具有显著的抑制作用。藻褐素对α-葡萄糖苷酶和α-淀粉酶的 IC50 值分别为 0.24 和 0.66mM，其活性均高于阿卡波糖。藻褐素在各种浓度（0.33-2.69mM）下对人脐静脉内皮细胞（HUVECs）均没有细胞毒性作用。与链脲佐菌素诱导的糖尿病或正常小鼠相比，给予藻褐素后，餐后血糖水平的升高明显受到抑制。此外，糖尿病小鼠的曲线下面积（AUC）通过给予藻褐素而显著降低（259 与 483mmol·min/L），并且它延迟了膳食碳水化合物的吸收。因此，这些结果表明，藻褐素可能是α-葡萄糖苷酶和α-淀粉酶的有效抑制剂。
