Kamine J, Rubin H
J Cell Physiol. 1977 Jul;92(1):1-11. doi: 10.1002/jcp.1040920102.
Using collagenase digestion as an assay for collagen in partially synchronized secondary cultures of chick embryo fibroblasts, we find that the rate of collagen synthesis remains at a constant fraction of overall protein synthesis (5%) regardless of the growth rate of the cells even when the rate of protein synthesis is accelerated 5-fold by adding serum and altering the pH of the culture medium. However, in cells oncogenically transformed by Rous sarcoma virus, the relative rate of collagen synthesis was decreased by 50% 24 hours after infection and was 10% of the initial rate after 5 days. This selective decrease in rate of collagen synthesis could be reversed in cells infected with an RSV temperature-sensitive transformation-defective mutant at the non-permissive temperature, indicating that the decrease in the rate of collagen synthesis was not merely the result of viral infection but was a direct consequence of oncogenic transformation.
利用胶原酶消化法测定鸡胚成纤维细胞部分同步化二级培养物中的胶原蛋白，我们发现，无论细胞的生长速率如何，胶原蛋白合成速率始终占总蛋白质合成的固定比例（5%），即便通过添加血清和改变培养基pH值使蛋白质合成速率加快5倍时也是如此。然而，在经劳斯肉瘤病毒致癌转化的细胞中，感染后24小时胶原蛋白合成的相对速率降低了50%，5天后降至初始速率的10%。在非允许温度下感染了劳斯肉瘤病毒温度敏感型转化缺陷突变体的细胞中，胶原蛋白合成速率的这种选择性降低可以逆转，这表明胶原蛋白合成速率的降低不仅仅是病毒感染的结果，而是致癌转化的直接后果。
