Heron I, Moller-Larsen A, Berg K
Infect Immun. 1977 Apr;16(1):48-53. doi: 10.1128/iai.16.1.48-53.1977.
Experiments were performed to analyze the characteristics of the effector cells involved in cell-mediated killing of herpes-infected fibroblasts. Effector cells were fractionated according to adherence and phagocytic properties, and lymphocytes were depleted of T-cells, and EA- and EAC- (antibody-coated bovine erythrocytes and bovine erythrocytes coated with rabbit anti-bovine antibodies and C5-deficient mouse serum, respectively) rosette-forming cells by differential centrifugation. Column passage was used as an alternative to depletion of B-cells and F(c)-receptor-positive cells. Antibody-dependent, cell-mediated cytotoxicity was found to be associated with nonphagocytic cells carrying low-avidity F(c) receptors. Some of these had surface immunoglobulin; others lacked both B- and T-cell markers. Low levels of killing were found without addition of anti-herpes antibody to the test system, and this killing seemed to depend on cells belonging to the same subpopulation as the antibody-dependent effector cells.
进行了实验以分析参与细胞介导杀伤疱疹感染成纤维细胞的效应细胞的特性。根据黏附性和吞噬特性对效应细胞进行分级分离，通过差速离心去除淋巴细胞中的T细胞以及EA和EAC（分别为抗体包被的牛红细胞以及用兔抗牛抗体和C5缺陷型小鼠血清包被的牛红细胞）花环形成细胞。采用柱层析法作为去除B细胞和F(c)受体阳性细胞的替代方法。发现抗体依赖性细胞介导的细胞毒性与携带低亲和力F(c)受体的非吞噬细胞有关。其中一些细胞有表面免疫球蛋白；其他细胞则缺乏B细胞和T细胞标记物。在测试系统中不添加抗疱疹抗体时发现杀伤水平较低，这种杀伤似乎取决于与抗体依赖性效应细胞属于同一亚群的细胞。
