Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
J Oral Rehabil. 2009 Jul;36(7):491-7. doi: 10.1111/j.1365-2842.2009.01964.x. Epub 2009 May 28.
To investigate the effect of the home use of a disclosing agent on the microbial composition of denture biofilm, by means of a cross-over randomized clinical trial. Two interventions were tested during 7 days each: (I) oral and denture hygiene instructions and (II) instructions associated with the home use of a disclosing agent (1% neutral red). Eleven participants with visible biofilm deposits over their maxillary complete dentures were randomly assigned to one of the two sequences of interventions: (i) I followed by II, and (ii) II followed by I. A washout period of 7 days was established. After each intervention, samples of denture biofilm were evaluated by DNA checkerboard hybridization for the detection of Candida spp. and 17 bacterial species. Counts were low for all the tested species, and no significant difference was found between the tested interventions (Wilcoxon test, P > 0.05). The home use of a disclosing agent does not remarkably change the composition of denture biofilm.
为了通过交叉随机临床试验研究家用显色剂对义齿生物膜微生物组成的影响。在 7 天内测试了两种干预措施：（I）口腔和义齿卫生指导，（II）与家用显色剂（1%中性红）使用相关的指导。11 名上颌全口义齿上有可见生物膜沉积物的参与者被随机分配到两种干预序列之一：（i）I 后接 II，和（ii）II 后接 I。建立了 7 天的洗脱期。在每次干预后，通过 DNA 斑点杂交检测义齿生物膜样本中的念珠菌属和 17 种细菌。所有测试的物种数量都较低，且两种测试干预措施之间没有发现显著差异（Wilcoxon 检验，P＞0.05）。家用显色剂的使用并不会显著改变义齿生物膜的组成。
