Badaró Maurício Malheiros, Salles Marcela Moreira, Leite Vanessa Maria Fagundes, Arruda Carolina Noronha Ferraz de, Oliveira Viviane de Cássia, Nascimento Cássio do, Souza Raphael Freitas de, Paranhos Helena de Freitas de Oliveira, Silva-Lovato Cláudia Helena
Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Materiais Dentários e Prótese, Ribeirão Preto, SP, Brazil.
J Appl Oral Sci. 2017 May-Jun;25(3):324-334. doi: 10.1590/1678-7757-2016-0222.
This study evaluated Ricinus communis and sodium hypochlorite solutions in terms of biofilm removal ability, remission of candidiasis, antimicrobial activity, and participant satisfaction.
It was conducted a controlled clinical trial, randomized, double-blind, and crossover. Sixty-four denture wearers with (n=24) and without candidiasis (n=40) were instructed to brush (3 times/day) and immerse their dentures (20 min/day) in different storage solutions (S1 / S2: 0.25% / 0.5% sodium hypochlorite; S3: 10% R. communis; S4: Saline).The trial period for each solution was seven days and a washout period of seven days was used before starting the use of another solution. The variables were analyzed at baseline and after each trial period. The biofilm of inner surfaces of maxillary dentures was disclosed, photographed, and total and dyed areas were measured (Image Tool software). The percentage of biofilm was calculated. Remission of candidiasis was assessed by visual scale and score were attributed. Antimicrobial activity was assessed by the DNA-Checkerboard hybridization method. Patient satisfaction was measured using a questionnaire.
S1 (4.41±7.98%) and S2 (2.93±5.23%) were more effective then S3 (6.95±10.93%) in biofilm remotion(P<0.0001). All solutions were different from the control (11.07±11.99%). S3 was the most effective solution in remission of candidiasis (50%), followed by S1 (46%). Concerning antimicrobial action, S1/S2 were similar and resulted in the lowest microorganism mean count (P=0.04), followed by S3. No significant differences were found with patient's satisfaction.
10% R. communis and 0.25% sodium hypochlorite were effective in biofilm removal, causing remission of candidiasis and reducing the formation of microbial colonies in denture surfaces. All solutions were approved by patients.
本研究从生物膜清除能力、念珠菌病缓解情况、抗菌活性和参与者满意度方面评估蓖麻和次氯酸钠溶液。
进行了一项对照临床试验，随机、双盲且交叉。64名佩戴假牙者，其中有念珠菌病的24名，无念珠菌病的40名，被指导每天刷牙3次，并将假牙每天浸泡20分钟于不同的储存溶液中（S1/S2：0.25%/0.5%次氯酸钠；S3：10%蓖麻；S4：生理盐水）。每种溶液的试验期为7天，在开始使用另一种溶液前有7天的洗脱期。在基线和每个试验期后分析变量。对上颌假牙内表面的生物膜进行显色、拍照，并测量总面积和染色面积（图像工具软件）。计算生物膜的百分比。通过视觉量表评估念珠菌病的缓解情况并给出评分。通过DNA棋盘杂交法评估抗菌活性。使用问卷测量患者满意度。
在生物膜清除方面，S1（4.41±7.98%）和S2（2.93±5.23%）比S3（6.95±10.93%）更有效（P<0.0001）。所有溶液均与对照组（11.07±11.99%）不同。S3是念珠菌病缓解方面最有效的溶液（50%），其次是S1（46%）。关于抗菌作用，S1/S2相似，导致微生物平均计数最低（P=0.04），其次是S3。患者满意度方面未发现显著差异。
10%蓖麻和0.25%次氯酸钠在清除生物膜、使念珠菌病缓解以及减少假牙表面微生物菌落形成方面有效。所有溶液均得到患者认可。
