美国犬猫肠球菌的流行情况、种属分布及耐药性分析。
Prevalence, species distribution and antimicrobial resistance of enterococci isolated from dogs and cats in the United States.
机构信息
Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA-ARS, Russell Research Center, Athens, 950 College Station Road, Athens, GA 30605, USA.
出版信息
J Appl Microbiol. 2009 Oct;107(4):1269-78. doi: 10.1111/j.1365-2672.2009.04310.x. Epub 2009 Apr 9.
AIMS
The contribution of dogs and cats as reservoirs of antimicrobial resistant enterococci remains largely undefined. This is increasingly important considering the possibility of transfer of bacteria from companion animals to the human host. In this study, dogs and cats from veterinary clinics were screened for the presence of enterococci.
METHODS AND RESULTS
A total of 420 enterococci were isolated from nasal, teeth, rectal, belly and hindquarters sites of 155 dogs and 121 cats from three clinics in Athens, GA. Eighty per cent (124 out of 155) of the dogs and 60% (72 out of 121) of the cats were positive for enterococci. From the total number of dog samples (n = 275), 32% (n = 87) were from hindquarter, 31% (n = 86) were rectal, and 29% (n = 79) were from the belly area. The majority of isolates originated from rectal samples (53 out of 145; 37%) from cats. The predominant species identified was Enterococcus faecalis (105 out of 155; 68%) from dogs and E. hirae (63 out of 121; 52%) from cats. Significantly more E. faecalis were isolated from rectal samples than any other enterococcal species (P < 0.05) for both dogs and cats suggesting site specific colonization of enterococcal species. The highest levels of resistance were to ciprofloxacin in E. faecium (9 out of 10; 90%), chloramphenicol resistance in E. faecalis (17 out of 20; 85%) and gentamicin resistance in E. faecalis (19 out of 24; 79%) from dog samples and nitrofurantoin resistance in E. faecium (15 out of 19; 79%) from cats. Multi-drug resistance (MDR) (resistance > or =2 antimicrobials) was observed to as few as two and as many as eight antimicrobials regardless of class.
CONCLUSION
This study demonstrated that dogs and cats are commonly colonized with antimicrobial resistant enterococci.
SIGNIFICANCE AND IMPACT OF THE STUDY
Dogs and cats may act as reservoirs of antimicrobial resistance genes that can be transferred from pets to people.
目的
犬和猫作为耐抗生素肠球菌的宿主的作用在很大程度上尚未确定。考虑到从伴侣动物向人类宿主转移细菌的可能性，这一点变得越来越重要。在这项研究中，从佐治亚州雅典的三家诊所的 155 只狗和 121 只猫的鼻腔、牙齿、直肠、腹部和臀部部位筛选出肠球菌。
方法和结果
从三只诊所的 155 只狗和 121 只猫的鼻腔、牙齿、直肠、腹部和臀部部位共分离出 420 株肠球菌。80%（124 只中的 155 只）的狗和 60%（72 只中的 121 只）的猫呈肠球菌阳性。在总狗样本数（n=275）中，32%（n=87）来自后肢，31%（n=86）来自直肠，29%（n=79）来自腹部。大多数分离株源自猫的直肠样本（53 个中的 145 个；37%）。鉴定的主要菌种是狗的粪肠球菌（155 个中的 105 个；68%）和猫的海氏肠球菌（121 个中的 63 个；52%）。从狗和猫的直肠样本中分离出的屎肠球菌明显多于任何其他肠球菌（P < 0.05），这表明肠球菌种的特定部位定植。在狗样本中，屎肠球菌对环丙沙星的耐药性最高（10 个中的 9 个；90%），粪肠球菌对氯霉素的耐药性最高（20 个中的 17 个；85%），粪肠球菌对庆大霉素的耐药性最高（24 个中的 19 个；79%），而在猫样本中，屎肠球菌对呋喃妥因的耐药性最高（19 个中的 15 个；79%）。无论药物种类如何，都观察到耐多种药物（MDR）（耐药性>或=2 种抗生素），少至两种，多至八种抗生素。
结论
本研究表明，狗和猫通常定植有耐抗生素肠球菌。
研究的意义和影响
狗和猫可能是携带可从宠物转移到人类的抗生素耐药基因的储主。
Zotero 插件