Zhao Yang, Zhou Xin, Chen Hua-Guo, Gong Xiao-Jian, Cai Zong-Wei, Zhou Chan-Yuan
The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China.
Fitoterapia. 2009 Oct;80(7):415-20. doi: 10.1016/j.fitote.2009.05.017. Epub 2009 May 30.
A simple, sensitive and accurate liquid chromatographic method with photodiode-array detection was developed for determination of dehydroevodiamine with detection wavelength at 368 nm and column temperature at 30 degrees C. The separation was carried out on an Agilent Zorbax SB-C(18) column (250 mm x 4.6 mm, 5 microm) together with a C(18) guard column. The mobile phase was acetonitrile-water (containing 30 mM sodium acetate trihydrate and 0.15% acetic acid) in the ratio of 30:70 (v/v) delivered at a flow rate of 1 mL/min. Excellent linear behavior was observed over the concentration range investigated, with correlation coefficient (R(2))=0.9998. This validated method was applied to determine the contents of dehydroevodiamine in 36 samples from different regions of China, and hierarchical clustering analysis was firstly used to classify and differentiate Evodia rutaecarpa samples. The analysis is specific and can be successfully applied to analyze E. rutaecarpa which is helpful for quality control of the herb.
建立了一种简单、灵敏且准确的带有光电二极管阵列检测的液相色谱法，用于测定吴茱萸次碱，检测波长为368 nm，柱温为30℃。分离在安捷伦Zorbax SB-C(18)柱（250 mm×4.6 mm，5 µm）上进行，并配有C(18)保护柱。流动相为乙腈-水（含有30 mM三水合醋酸钠和0.15%醋酸），比例为30:70（v/v），流速为1 mL/min。在所研究的浓度范围内观察到了良好的线性关系，相关系数（R(2)）=0.9998。该验证方法用于测定来自中国不同地区的36个样品中吴茱萸次碱的含量，并且首次使用层次聚类分析对吴茱萸样品进行分类和区分。该分析具有特异性，可成功应用于吴茱萸的分析，有助于该草药的质量控制。
