Tan Xiao-mei, Peng Shu-ling
Key Research Laboratory of New Traditional Chinese Drugs, School of TCM, Southern Medical University, Guangzhou 510515, China.
Zhong Yao Cai. 2007 Mar;30(3):297-9.
To identify Eevodia rutaecarpa (Juss.) Benth and determine the contents of the total alkaloid of Rhizoma Coptidis in Yuhuanglian extract.
Thin layer chromatography (TLC) was used for the identification of Evodia rutaecarpa (Juss.) Benth with a mixed solvent of cyclohexane-ethyl acetate-methanol-triethylamine (19:5:1:1) as the developer and spraying with 10% sulfuric acid ethanol solution as colorimetric method. The contents of the total alkaloid of Rhizoma Coptidis was determined by UV spectrophometry in Yuhuanglian extract and the detection wavelength was 349 nm.
Special spots could be observed at 365 nm on positions corresponding to Evodia rutaecarpa (Juss.) Benth, and the preparation, while the negative control showed no spots. The linear range of berberine hydrochloric was 1.04-11.44 microg/ml, r = 0.9999; the average recovery was 99.2%, and RSD was 1.0% (n=5).
Evodia rutaecarpa (Juss.) Benth can be identified simply and fast using TLC and the method to determine the contents of the total alkalid of Rhizoma Coptidis by UV spectrophometry is accurate, rapid and simple. It can be used for quality control of Yuhuanglian extract.
鉴别萸黄连提取物中的吴茱萸，并测定其中黄连总生物碱的含量。
采用薄层色谱法（TLC）鉴别吴茱萸，以环己烷 - 乙酸乙酯 - 甲醇 - 三乙胺（19:5:1:1）混合溶剂为展开剂，喷以10%硫酸乙醇溶液显色。采用紫外分光光度法测定萸黄连提取物中黄连总生物碱的含量，检测波长为349nm。
在与吴茱萸对照品相应位置的365nm处可观察到特征斑点，供试品溶液有相同斑点，而阴性对照无斑点。盐酸小檗碱的线性范围为1.04 - 11.44μg/ml，r = 0.9999；平均回收率为99.2%，RSD为1.0%（n = 5）。
采用TLC法可简便快速地鉴别吴茱萸，紫外分光光度法测定黄连总生物碱含量的方法准确、快速、简便，可用于萸黄连提取物的质量控制。
