白细胞介素-12 基因多态性与宫颈癌风险。

Interleukin-12 gene polymorphism and cervical cancer risk.

机构信息

Department of Biotechnology, Panjab University, Chandigarh, India.

出版信息

Am J Clin Oncol. 2009 Oct;32(5):524-8. doi: 10.1097/COC.0b013e318192519a.

DOI:10.1097/COC.0b013e318192519a

PMID:19487916

Abstract

AIM

: To investigate the effect of interleukin-12 p40 gene (IL-12β) 3'-untranslated region polymorphism on the risk of cervical cancer.

METHODS

DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. Polymerase chain reaction restriction fragment length polymorphism was used for the detection of IL-12β (1188A/C) gene polymorphism.

RESULTS

It was observed that genotypes AC [odds ratio (OR) = 1.80, 95% confidence interval (CI) = 1.17-2.78] and AC + CC (OR = 1.71, 95% CI = 1.11-2.63, P = 0.01) increase the risk of cervical cancer. The strength of the risk in passive smokers with AC and AC + CC genotypes was increased to more than 2.8 times in comparison with a nonsmoker with AA genotype (OR = 2.94, 95% CI = 1.56-5.55, P < 0.001; OR = 2.83, 95% CI = 1.52-5.31, P < 0.001, respectively).

CONCLUSION

This is the first study to provide an evidence for the association of IL-12β (1188A/C) gene polymorphism with the risk of cervical cancer.

摘要

目的

探讨白细胞介素 12 p40 基因（IL-12β）3′非翻译区多态性与宫颈癌发病风险的关系。

方法

提取 200 例宫颈癌患者和 200 例健康对照者外周血 DNA，采用聚合酶链反应-限制性片段长度多态性方法检测 IL-12β（1188A/C）基因多态性。

结果

观察到 AC 基因型[比值比（OR）=1.80，95%置信区间（CI）=1.17-2.78]和 AC+CC 基因型（OR=1.71，95%CI=1.11-2.63，P=0.01）增加了宫颈癌发病风险。与 AA 基因型的非吸烟者相比，AC 和 AC+CC 基因型的被动吸烟者患宫颈癌的风险分别增加了 2.8 倍以上（OR=2.94，95%CI=1.56-5.55，P<0.001；OR=2.83，95%CI=1.52-5.31，P<0.001）。