Han Seung-Su, Cho Eun-Young, Lee Taek Sang, Kim Jae Weon, Park Noh-Hyun, Song Yong-Sang, Kim Jung Gu, Lee Hyo-Pyo, Kang Soon-Beom
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, 28 Yongun-dong, Chongno-gu, Seoul 110-744, South Korea.
Eur J Obstet Gynecol Reprod Biol. 2008 Sep;140(1):71-5. doi: 10.1016/j.ejogrb.2008.02.007. Epub 2008 Mar 25.
The aim of this study was to investigate whether IL12B polymorphisms might be associated with increased risk and invasiveness of cervical cancer in Korean women.
Peripheral blood samples from patients with invasive cervical cancer (n=154) and non-cancer controls (n=191) were used to detect three biallelic IL12B polymorphisms at IVS2 -912, IVS4 +314, 3'UTR +1188 sites by performing SNaPshot assay. Allelic frequencies, genotype distributions, and haplotype patterns in the case group were compared with those in the control group. The relationships between these polymorphisms and cancer invasiveness were also evaluated by collating the clinicopathologic parameters including FIGO stage, lymph node status, histologic type, and parametrial invasion. The used analytic methods are chi-square test and logistic regression analysis.
Allelic frequencies of cases (G, 0.853; A, 0.147) were not significantly different from controls (G, 0.796; A, 0.204) in IVS2 -912G/A SNP (P=0.054). GG genotype of IVS2 -912G/A SNP showed increased risk for cervical cancer compared with AA genotype (P=0.040). The IVS2 -912G:IVS4+314A haplotype, IVS2 -912G:IVS4 +314A:3'UTR +1188A haplotype, and IVS2 -912G:IVS4 +314A:3'UTR +1188C haplotype were also significantly associated with increased risk for cervical cancer. A subgroup analysis of the clinicopathologic parameters in cancer group also showed that there is no significant association between IL12B polymorphisms and cervical cancer invasiveness.
This study suggests that IVS2 -912GG genotype and IVS2 -912G:IVS4 +314A haplotype of IL12B gene are associated with increased risk for cervical cancer in Korean women.
本研究旨在调查白细胞介素12B（IL12B）基因多态性是否可能与韩国女性宫颈癌风险增加及侵袭性增强相关。
采用SNaPshot检测法，对154例浸润性宫颈癌患者和191例非癌症对照者的外周血样本进行检测，以确定IL12B基因在IVS2 -912、IVS4 +314、3'UTR +1188位点的三个双等位基因多态性。比较病例组和对照组的等位基因频率、基因型分布及单倍型模式。通过整理包括国际妇产科联盟（FIGO）分期、淋巴结状态、组织学类型和宫旁组织浸润等临床病理参数，评估这些多态性与癌症侵袭性之间的关系。所采用的分析方法为卡方检验和逻辑回归分析。
在IVS2 -912G/A单核苷酸多态性（SNP）中，病例组（G，0.853；A，0.147）的等位基因频率与对照组（G，0.796；A，0.204）无显著差异（P = 0.054）。与AA基因型相比，IVS2 -912G/A SNP的GG基因型显示宫颈癌风险增加（P = 0.040）。IVS2 -912G:IVS4+314A单倍型、IVS2 -912G:IVS4 +314A:3'UTR +1188A单倍型以及IVS2 -912G:IVS4 +314A:3'UTR +1188C单倍型也与宫颈癌风险增加显著相关。癌症组临床病理参数的亚组分析还显示，IL12B基因多态性与宫颈癌侵袭性之间无显著关联。
本研究表明，IL12B基因的IVS2 -912GG基因型和IVS2 -912G:IVS4 +314A单倍型与韩国女性宫颈癌风险增加相关。
