Olivier Nicolas, Mermillod-Blondin Alexandre, Arnold Craig B, Beaurepaire Emmanuel
Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, F-91128 Palaiseau, France.
Opt Lett. 2009 Jun 1;34(11):1684-6. doi: 10.1364/ol.34.001684.
We describe a simple setup that allows depth of field switching at kilohertz rates in a nonlinear microscope. Beam profile and/or divergence are modulated using a tunable, acoustically driven gradient-index fluid lens. We demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and the other based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning, resulting in the interlaced acquisition of two different images. We apply this approach to the simultaneous standard and 4.5x-extended depth-of-field imaging of developing embryos.
我们描述了一种简单的装置，该装置可在非线性显微镜中以千赫兹速率进行景深切换。使用可调谐的、声学驱动的梯度折射率流体透镜对光束轮廓和/或发散度进行调制。我们展示了两种调制策略，一种基于每个像素期间的快速变焦扫描，另一种基于伪贝塞尔光束激发。在扫描过程中每行切换平均光束形状，从而交错采集两幅不同的图像。我们将这种方法应用于发育胚胎的同时标准和4.5倍扩展景深成像。
